A spectrophotometric method for calmodulin assay
The activity of calmodulin as an activator of cAMP phosphodiesterase was assayed. AMP was hydrolyzed by 5'-nucleotidase, and the adenosine formed was measured by both liquid scintillation counting and spectrophotometry at 265 nm. Calmodulin activities measured by the two methods were equivalent...
| Publié dans: | BioTechniques. - 1991. - 6(1988), 4 vom: 01. Apr., Seite 294-6 |
|---|---|
| Auteur principal: | |
| Autres auteurs: | , , , , , |
| Format: | Article |
| Langue: | English |
| Publié: |
1988
|
| Accès à la collection: | BioTechniques |
| Sujets: | Comparative Study Journal Article Calmodulin Cyclic AMP E0399OZS9N 3',5'-Cyclic-AMP Phosphodiesterases EC 3.1.4.17 |
| Résumé: | The activity of calmodulin as an activator of cAMP phosphodiesterase was assayed. AMP was hydrolyzed by 5'-nucleotidase, and the adenosine formed was measured by both liquid scintillation counting and spectrophotometry at 265 nm. Calmodulin activities measured by the two methods were equivalent, indicating that spectrophotometric assay of calmodulin can be used in place of the isotopic method |
|---|---|
| Description: | Date Completed 20.06.1990 Date Revised 21.11.2013 published: Print Citation Status MEDLINE |
| ISSN: | 1940-9818 |