A spectrophotometric method for calmodulin assay
The activity of calmodulin as an activator of cAMP phosphodiesterase was assayed. AMP was hydrolyzed by 5'-nucleotidase, and the adenosine formed was measured by both liquid scintillation counting and spectrophotometry at 265 nm. Calmodulin activities measured by the two methods were equivalent...
Publié dans: | BioTechniques. - 1991. - 6(1988), 4 vom: 01. Apr., Seite 294-6 |
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Auteur principal: | |
Autres auteurs: | , , , , , |
Format: | Article |
Langue: | English |
Publié: |
1988
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Accès à la collection: | BioTechniques |
Sujets: | Comparative Study Journal Article Calmodulin Cyclic AMP E0399OZS9N 3',5'-Cyclic-AMP Phosphodiesterases EC 3.1.4.17 |