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NLM02144062X |
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DE-627 |
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20250125115748.0 |
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tu |
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231221s1990 xx ||||| 00| ||eng c |
| 028 |
5 |
2 |
|a pubmed25n0072.xml
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|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Oommen, A
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Single-step labeling of DNA using restriction endonucleases and T4 polynucleotide kinase
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| 264 |
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1 |
|c 1990
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
|
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 01.08.1990
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| 500 |
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|a Date Revised 15.11.2006
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| 500 |
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|a published: Print
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| 500 |
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|a Citation Status MEDLINE
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| 520 |
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|a DNA restriction fragments can be end-labeled in a combined digestion and labeling reaction with restriction endonuclease plus T4 polynucleotide kinase, omitting a dephosphorylation step. Both pBR322 and phage lambda DNA digests are efficiently labeled in one step, one tube and one incubation
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| 650 |
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
|
7 |
|a DNA, Viral
|2 NLM
|
| 650 |
|
7 |
|a Phosphotransferases
|2 NLM
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| 650 |
|
7 |
|a EC 2.7.-
|2 NLM
|
| 650 |
|
7 |
|a Polynucleotide 5'-Hydroxyl-Kinase
|2 NLM
|
| 650 |
|
7 |
|a EC 2.7.1.78
|2 NLM
|
| 650 |
|
7 |
|a DNA Restriction Enzymes
|2 NLM
|
| 650 |
|
7 |
|a EC 3.1.21.-
|2 NLM
|
| 700 |
1 |
|
|a Ferrandis, I
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Wang, M J
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1988
|g 8(1990), 5 vom: 15. Mai, Seite 482, 484, 486
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
|
| 773 |
1 |
8 |
|g volume:8
|g year:1990
|g number:5
|g day:15
|g month:05
|g pages:482, 484, 486
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|d 8
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|h 482, 484, 486
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