Single-step labeling of DNA using restriction endonucleases and T4 polynucleotide kinase
DNA restriction fragments can be end-labeled in a combined digestion and labeling reaction with restriction endonuclease plus T4 polynucleotide kinase, omitting a dephosphorylation step. Both pBR322 and phage lambda DNA digests are efficiently labeled in one step, one tube and one incubation
| Veröffentlicht in: | BioTechniques. - 1988. - 8(1990), 5 vom: 15. Mai, Seite 482, 484, 486 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1990
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. DNA, Viral Phosphotransferases EC 2.7.- Polynucleotide 5'-Hydroxyl-Kinase EC 2.7.1.78 DNA Restriction Enzymes EC 3.1.21.- |
| Zusammenfassung: | DNA restriction fragments can be end-labeled in a combined digestion and labeling reaction with restriction endonuclease plus T4 polynucleotide kinase, omitting a dephosphorylation step. Both pBR322 and phage lambda DNA digests are efficiently labeled in one step, one tube and one incubation |
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| Beschreibung: | Date Completed 01.08.1990 Date Revised 15.11.2006 published: Print Citation Status MEDLINE |
| ISSN: | 0736-6205 |