A rapid method for recombination and site-specific mutagenesis by placing homologous ends on DNA using polymerase chain reaction
We have developed a novel polymerase chain reaction (PCR) method that permits the rapid generation of site-specific mutants and recombinant DNA constructs with a minimum number of steps and primers. DNA segments are modified by using amplifying primers that add homologous ends to the polymerase chai...
| Publié dans: | BioTechniques. - 1991. - 10(1991), 1 vom: 05. Jan., Seite 62-6 |
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| Format: | Article |
| Langue: | English |
| Publié: |
1991
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article DNA, Recombinant |