A rapid method for recombination and site-specific mutagenesis by placing homologous ends on DNA using polymerase chain reaction
We have developed a novel polymerase chain reaction (PCR) method that permits the rapid generation of site-specific mutants and recombinant DNA constructs with a minimum number of steps and primers. DNA segments are modified by using amplifying primers that add homologous ends to the polymerase chai...
| Veröffentlicht in: | BioTechniques. - 1991. - 10(1991), 1 vom: 05. Jan., Seite 62-6 |
|---|---|
| 1. Verfasser: | |
| Weitere Verfasser: | |
| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1991
|
| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article DNA, Recombinant |