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231221s1991 xx ||||| 00| ||eng c |
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|a eng
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| 100 |
1 |
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|a Zagursky, R J
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Use of 33P for Sanger DNA sequencing
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| 264 |
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1 |
|c 1991
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 02.01.1992
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| 500 |
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|a Date Revised 23.11.2016
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| 500 |
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a The use of [alpha-33P]deoxyadenosine 5'-triphosphate ([alpha-33P]dATP) in DNA sequencing has been described. 33P has a maximum beta-emission energy that is 50% stronger than 35S, but fivefold weaker than 32P. As a result, sequences generated using [alpha-33P]dATP have short exposure times like 32P, yet they maintain band resolution similar to 35S. Handling of [alpha-33P]dATP is straightforward because no special lead or Plexiglas shielding is necessary
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| 650 |
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4 |
|a Journal Article
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| 650 |
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7 |
|a Deoxyadenine Nucleotides
|2 NLM
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| 650 |
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7 |
|a Phosphorus Radioisotopes
|2 NLM
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| 650 |
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7 |
|a DNA
|2 NLM
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| 650 |
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7 |
|a 9007-49-2
|2 NLM
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| 650 |
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7 |
|a 2'-deoxyadenosine triphosphate
|2 NLM
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| 650 |
|
7 |
|a K8KCC8SH6N
|2 NLM
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| 700 |
1 |
|
|a Conway, P S
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kashdan, M A
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1988
|g 11(1991), 1 vom: 15. Juli, Seite 36, 38
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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| 773 |
1 |
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|g volume:11
|g year:1991
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|g pages:36, 38
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