Use of 33P for Sanger DNA sequencing

The use of [alpha-33P]deoxyadenosine 5'-triphosphate ([alpha-33P]dATP) in DNA sequencing has been described. 33P has a maximum beta-emission energy that is 50% stronger than 35S, but fivefold weaker than 32P. As a result, sequences generated using [alpha-33P]dATP have short exposure times like...

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Bibliographische Detailangaben
Veröffentlicht in:BioTechniques. - 1988. - 11(1991), 1 vom: 15. Juli, Seite 36, 38
1. Verfasser: Zagursky, R J (VerfasserIn)
Weitere Verfasser: Conway, P S, Kashdan, M A
Format: Aufsatz
Sprache:English
Veröffentlicht: 1991
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Deoxyadenine Nucleotides Phosphorus Radioisotopes DNA 9007-49-2 2'-deoxyadenosine triphosphate K8KCC8SH6N
Beschreibung
Zusammenfassung:The use of [alpha-33P]deoxyadenosine 5'-triphosphate ([alpha-33P]dATP) in DNA sequencing has been described. 33P has a maximum beta-emission energy that is 50% stronger than 35S, but fivefold weaker than 32P. As a result, sequences generated using [alpha-33P]dATP have short exposure times like 32P, yet they maintain band resolution similar to 35S. Handling of [alpha-33P]dATP is straightforward because no special lead or Plexiglas shielding is necessary
Beschreibung:Date Completed 02.01.1992
Date Revised 23.11.2016
published: Print
Citation Status MEDLINE
ISSN:0736-6205