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|a eng
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| 100 |
1 |
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|a Sarkar, G
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Parameters affecting susceptibility of PCR contamination to UV inactivation
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| 264 |
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1 |
|c 1991
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 31.10.1991
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| 500 |
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|a Date Revised 17.11.2004
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| 500 |
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a Contamination of reagents used for PCR is a serious problem. We have recently reported the remarkable effectiveness of UV light in successful decontamination of PCR reagents when the reagents were contaminated with a 6-kb plasmid, followed by amplification of 750-bp segment from the insert. However, further investigation reveals that segment size, sequence and hydration can have a dramatic effect on the efficiency of UV inactivation. Despite some limitations, UV remains a highly effective means of decontamination
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| 650 |
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|a Journal Article
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7 |
|a Factor IX
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| 650 |
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|a DNA
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| 650 |
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|a 9007-49-2
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| 700 |
1 |
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|a Sommer, S S
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1991
|g 10(1991), 5 vom: 01. Mai, Seite 590-4
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnas
|
| 773 |
1 |
8 |
|g volume:10
|g year:1991
|g number:5
|g day:01
|g month:05
|g pages:590-4
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