Parameters affecting susceptibility of PCR contamination to UV inactivation
Contamination of reagents used for PCR is a serious problem. We have recently reported the remarkable effectiveness of UV light in successful decontamination of PCR reagents when the reagents were contaminated with a 6-kb plasmid, followed by amplification of 750-bp segment from the insert. However,...
| Publié dans: | BioTechniques. - 1991. - 10(1991), 5 vom: 01. Mai, Seite 590-4 |
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| Auteur principal: | |
| Autres auteurs: | |
| Format: | Article |
| Langue: | English |
| Publié: |
1991
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| Accès à la collection: | BioTechniques |
| Sujets: | Journal Article Factor IX 9001-28-9 DNA 9007-49-2 |
| Résumé: | Contamination of reagents used for PCR is a serious problem. We have recently reported the remarkable effectiveness of UV light in successful decontamination of PCR reagents when the reagents were contaminated with a 6-kb plasmid, followed by amplification of 750-bp segment from the insert. However, further investigation reveals that segment size, sequence and hydration can have a dramatic effect on the efficiency of UV inactivation. Despite some limitations, UV remains a highly effective means of decontamination |
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| Description: | Date Completed 31.10.1991 Date Revised 17.11.2004 published: Print Citation Status MEDLINE |
| ISSN: | 1940-9818 |