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231221s1991 xx ||||| 00| ||eng c |
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|a DE-627
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|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Straus, N A
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a In vitro production of large single-stranded templates for DNA sequencing
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| 264 |
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1 |
|c 1991
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ohne Hilfsmittel zu benutzen
|b n
|2 rdamedia
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| 338 |
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|a Band
|b nc
|2 rdacarrier
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| 500 |
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|a Date Completed 14.08.1991
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| 500 |
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|a Date Revised 15.11.2006
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| 500 |
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|a published: Print
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| 500 |
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|a Citation Status MEDLINE
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| 520 |
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|a A method has been developed for the preparation of large single-stranded DNA sequencing templates from primary cloning plasmids or cosmids. The method utilizes the separate action of T7 Gene 6 exonuclease and exonuclease III to generate large quantities of single-stranded template for each strand of a large-cloned fragment. Since the procedure is intended for use on primary clones, it avoids the time-consuming subcloning steps associated with most sequencing programs. The procedure also has the advantage of avoiding clone instability problems associated with subcloning in M13
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| 650 |
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4 |
|a Journal Article
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| 650 |
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7 |
|a DNA, Recombinant
|2 NLM
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| 650 |
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7 |
|a DNA, Single-Stranded
|2 NLM
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| 650 |
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7 |
|a Exodeoxyribonucleases
|2 NLM
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| 650 |
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7 |
|a EC 3.1.-
|2 NLM
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| 650 |
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7 |
|a gene b exonuclease
|2 NLM
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| 650 |
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7 |
|a EC 3.1.11.-
|2 NLM
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| 650 |
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7 |
|a exodeoxyribonuclease III
|2 NLM
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| 650 |
|
7 |
|a EC 3.1.11.2
|2 NLM
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| 650 |
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7 |
|a DNA Restriction Enzymes
|2 NLM
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| 650 |
|
7 |
|a EC 3.1.21.-
|2 NLM
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| 700 |
1 |
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|a Zagursky, R J
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1988
|g 10(1991), 3 vom: 15. März, Seite 376-84
|w (DE-627)NLM012627046
|x 0736-6205
|7 nnns
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| 773 |
1 |
8 |
|g volume:10
|g year:1991
|g number:3
|g day:15
|g month:03
|g pages:376-84
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