In vitro production of large single-stranded templates for DNA sequencing
A method has been developed for the preparation of large single-stranded DNA sequencing templates from primary cloning plasmids or cosmids. The method utilizes the separate action of T7 Gene 6 exonuclease and exonuclease III to generate large quantities of single-stranded template for each strand of...
| Veröffentlicht in: | BioTechniques. - 1988. - 10(1991), 3 vom: 15. März, Seite 376-84 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1991
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article DNA, Recombinant DNA, Single-Stranded Exodeoxyribonucleases EC 3.1.- gene b exonuclease EC 3.1.11.- exodeoxyribonuclease III EC 3.1.11.2 DNA Restriction Enzymes |
| Zusammenfassung: | A method has been developed for the preparation of large single-stranded DNA sequencing templates from primary cloning plasmids or cosmids. The method utilizes the separate action of T7 Gene 6 exonuclease and exonuclease III to generate large quantities of single-stranded template for each strand of a large-cloned fragment. Since the procedure is intended for use on primary clones, it avoids the time-consuming subcloning steps associated with most sequencing programs. The procedure also has the advantage of avoiding clone instability problems associated with subcloning in M13 |
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| Beschreibung: | Date Completed 14.08.1991 Date Revised 15.11.2006 published: Print Citation Status MEDLINE |
| ISSN: | 0736-6205 |