Exonuclease cycling assay : an amplified assay for the detection of specific DNA sequences
An assay is described in which an oligonucleotide probe is specifically digested by lambda exonuclease only when it is annealed to its complementary sequence. In this assay, a cycling effect occurs whereby a small amount of target sequence acts as a specific co-factor in the enzymatic degradation of...
| Veröffentlicht in: | BioTechniques. - 1988. - 13(1992), 6 vom: 12. Dez., Seite 888-92 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1992
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Oligonucleotide Probes Viral Proteins DNA 9007-49-2 Exodeoxyribonucleases EC 3.1.- exo protein, Bacteriophage lambda EC 3.1.11.3 |
| Zusammenfassung: | An assay is described in which an oligonucleotide probe is specifically digested by lambda exonuclease only when it is annealed to its complementary sequence. In this assay, a cycling effect occurs whereby a small amount of target sequence acts as a specific co-factor in the enzymatic degradation of a larger number of molecules of an oligonucleotide probe. This amplification principle is demonstrated and the effect of the oligonucleotide probe sequence investigated. The necessary steps needed to convert this effect into a useful diagnostic tool are discussed |
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| Beschreibung: | Date Completed 11.02.1993 Date Revised 10.12.2019 published: Print Citation Status MEDLINE |
| ISSN: | 0736-6205 |