Exonuclease cycling assay : an amplified assay for the detection of specific DNA sequences

An assay is described in which an oligonucleotide probe is specifically digested by lambda exonuclease only when it is annealed to its complementary sequence. In this assay, a cycling effect occurs whereby a small amount of target sequence acts as a specific co-factor in the enzymatic degradation of...

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Veröffentlicht in:BioTechniques. - 1988. - 13(1992), 6 vom: 12. Dez., Seite 888-92
1. Verfasser: Copley, C G (VerfasserIn)
Weitere Verfasser: Boot, C
Format: Aufsatz
Sprache:English
Veröffentlicht: 1992
Zugriff auf das übergeordnete Werk:BioTechniques
Schlagworte:Journal Article Oligonucleotide Probes Viral Proteins DNA 9007-49-2 Exodeoxyribonucleases EC 3.1.- exo protein, Bacteriophage lambda EC 3.1.11.3