A simple and rapid method for screening transformant yeast colonies using PCR
A simple and rapid procedure for screening transformant yeast colonies is described. In this method, a trace amount of plasmid DNA is isolated from a small amount of yeast cell mass; then, the presence of the exogenous DNA in each yeast colony is detected by PCR amplification
| Veröffentlicht in: | BioTechniques. - 1988. - 13(1992), 5 vom: 01. Nov., Seite 730-2, 735 |
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| Format: | Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
1992
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| Zugriff auf das übergeordnete Werk: | BioTechniques |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't DNA, Recombinant Lactoferrin EC 3.4.21.- |
| Zusammenfassung: | A simple and rapid procedure for screening transformant yeast colonies is described. In this method, a trace amount of plasmid DNA is isolated from a small amount of yeast cell mass; then, the presence of the exogenous DNA in each yeast colony is detected by PCR amplification |
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| Beschreibung: | Date Completed 10.12.1992 Date Revised 17.11.2011 published: Print Citation Status MEDLINE |
| ISSN: | 1940-9818 |