A simple and rapid method for screening transformant yeast colonies using PCR

A simple and rapid procedure for screening transformant yeast colonies is described. In this method, a trace amount of plasmid DNA is isolated from a small amount of yeast cell mass; then, the presence of the exogenous DNA in each yeast colony is detected by PCR amplification

Détails bibliographiques
Publié dans:BioTechniques. - 1991. - 13(1992), 5 vom: 01. Nov., Seite 730-2, 735
Auteur principal: Liang, Q (Auteur)
Autres auteurs: Richardson, T
Format: Article
Langue:English
Publié: 1992
Accès à la collection:BioTechniques
Sujets:Journal Article Research Support, Non-U.S. Gov't DNA, Recombinant Lactoferrin EC 3.4.21.-