A simple and rapid method for screening transformant yeast colonies using PCR
A simple and rapid procedure for screening transformant yeast colonies is described. In this method, a trace amount of plasmid DNA is isolated from a small amount of yeast cell mass; then, the presence of the exogenous DNA in each yeast colony is detected by PCR amplification
Publié dans: | BioTechniques. - 1991. - 13(1992), 5 vom: 01. Nov., Seite 730-2, 735 |
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Auteur principal: | |
Autres auteurs: | |
Format: | Article |
Langue: | English |
Publié: |
1992
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Accès à la collection: | BioTechniques |
Sujets: | Journal Article Research Support, Non-U.S. Gov't DNA, Recombinant Lactoferrin EC 3.4.21.- |