Single-cell transcriptomics reveals the functional trajectories of the endocarp cell clusters in the postharvest fruit senescence process of Hylocereus undatus
Copyright © 2025 Elsevier Masson SAS. All rights reserved.
| Veröffentlicht in: | Plant physiology and biochemistry : PPB. - 1991. - 229(2025), Pt C vom: 11. Okt., Seite 110623 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2025
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| Zugriff auf das übergeordnete Werk: | Plant physiology and biochemistry : PPB |
| Schlagworte: | Journal Article Endocarp Flavonoids Fruit senescence Gene regulatory network (GRN) Pseudotime Single-cell transcriptomics |
| Zusammenfassung: | Copyright © 2025 Elsevier Masson SAS. All rights reserved. Fruit senescence is a complex physiological process. In this study, we employed single-cell transcriptomics combined with phenotypic and functional analyses to investigate the role of endocarp cell clusters in postharvest senescence of Hylocereus undatus. Morphological changes of the endocarp were recorded, the temporal dynamics of superoxide anion production and flavonoid biosynthesis were characterized, and overall ROS accumulation was visualized by fluorescence microscopy. Single-cell RNA sequencing identified three endocarp-specific cell clusters (ScC4, ScC11, and ScC12), which were respectively enriched in antioxidant defense, secondary metabolism, and hormone signaling pathways. Pseudotime trajectory analysis revealed that ScC4 acted as the initiating stress-responsive cells, ScC11 mediated flavonoid biosynthesis and ROS scavenging, while ScC12 functioned as late-stage signaling cells associated with abscisic acid and ethylene pathways. Gene regulatory network inference further identified HuGIP2 as a central hub gene in ScC11. Virus-induced gene silencing demonstrated that suppression of HuGIP2 reduced flavonoid accumulation, increased ROS levels, enhanced lipoxygenase (LOX) activity, and accelerated senescence. Collectively, this study delineates the differentiation trajectories of endocarp cell populations during fruit senescence and proposes a mechanistic hypothesis whereby flavonoid-mediated ROS scavenging and LOX inhibition play pivotal roles in delaying senescence. These findings provide single-cell-level insights and spatiotemporal perspectives into the dynamic process of plant senescence |
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| Beschreibung: | Date Revised 18.10.2025 published: Print-Electronic Citation Status Publisher |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2025.110623 |