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|a 10.1093/jxb/eraf408
|2 doi
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|a DE-627
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|a eng
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|a Zhang, Jie
|e verfasserin
|4 aut
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|a A V232M missense mutation in rice OsLSZ1 inhibits β-carotene biosynthesis and impairs chloroplast development during seedling stage at low temperatures
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|c 2025
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|a Text
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|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|a Date Revised 15.10.2025
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|a published: Print-Electronic
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|a Citation Status Publisher
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|a © The Author(s) 2025. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For commercial re-use, please contact reprintsoup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.
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|a Carotenoids are essential for chloroplast development, with lycopene β-cyclase as the rate-limiting enzyme in the biosynthesis of β-carotene and α-carotene. This study investigates the carotenoid biosynthetic alterations in a low-temperature-sensitive zebra-leaf rice mutant oslsz1, which exhibits yellow or yellow-green striped leaves before 5-leaf stage at lower temperatures (22 °C and 28 °C) but develops normally at higher temperature (34 °C). Metabolomics revealed that at 22 °C, levels of β-carotene and xanthophylls decreased, while levels of (E/Z)-phytoene, lycopene, α-carotene and γ-carotene increased in oslsz1 as compared to the wild type. Transmission electron microscopy demonstrated defective chloroplast development in oslsz1 at 22 °C. Through map-based cloning, the causative gene was identified as OsLCYb that encodes lycopene β-cyclase with a V232M missense mutation. mRNA quantification showed that OsLSZ1/OsLCYb is ubiquitously expressed, especially in young-seedling leaves, with suppression at low temperatures. Heterologous expression of oslsz1/oslcyb in Escherichia coli confirmed a near-complete loss of lycopene conversion to β-carotene at 22 °C. Further domain dissection indicated that the N-terminal 181 amino-acids of OsLSZ1/OsLCYb are critical for low-temperature sensitivity, while its C-terminal extension enhances this response. In summary, these findings underscore the crucial role of OsLSZ1/OsLCYb in carotenoid biosynthesis and chloroplast development in rice at low temperatures
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|a Journal Article
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|a OsLSZ1/OsLCYb
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|a Rice
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|a chloroplast development
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|a enzymatic function
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|a low-temperature sensitive
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|a lycopene β-cyclase
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|a map-based cloning
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|a β-Carotene
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|a Zhao, Jiayi
|e verfasserin
|4 aut
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|a Chen, Jiameng
|e verfasserin
|4 aut
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| 700 |
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|a Cai, Chenying
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Huang, Fudeng
|e verfasserin
|4 aut
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| 700 |
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|a Cheng, Fangmin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Pan, Gang
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g (2025) vom: 15. Okt.
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnas
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| 773 |
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|g year:2025
|g day:15
|g month:10
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|u http://dx.doi.org/10.1093/jxb/eraf408
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