Reassessment of Myxobolus toyamai Morphology in Koi Carp Using an Enhanced Giemsa Warming Technique : A First Report From Vietnam
© 2025 John Wiley & Sons Ltd.
| Veröffentlicht in: | Journal of fish diseases. - 1998. - 48(2025), 11 vom: 03. Okt., Seite e14152 |
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| Weitere Verfasser: | , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2025
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| Zugriff auf das übergeordnete Werk: | Journal of fish diseases |
| Schlagworte: | Journal Article Giemsa warming method histological staining intralamellar vascular myxosporean respiratory impairment severe gill lesions unequal polar capsules RNA, Ribosomal, 18S |
| Zusammenfassung: | © 2025 John Wiley & Sons Ltd. Myxobolus toyamai, a well-established gill parasite of common carp, Cyprinus carpio, has been implicated in infections across multiple continents. Despite numerous attempts to classify this parasite based on the structural features of its polar capsules, existing staining techniques have proven inadequate, resulting in unclear structural definitions. This study represents the first report of M. toyamai infection in koi fish in Vietnam, along with the optimisation of a staining technique using Giemsa warming to accurately define its morphological characteristics. In addition, the study includes the analysis of the 18S rRNA gene and histopathological evaluations. The results show that the Giemsa warming staining method effectively clarifies the structure of the polar capsules, revealing distinct measurements that differ from those reported in previous studies. Furthermore, sequence analysis of the 18S rDNA gene demonstrates a high degree of similarity to M. toyamai, confirming its identification in the examined specimens |
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| Beschreibung: | Date Completed 02.10.2025 Date Revised 02.10.2025 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1365-2761 |
| DOI: | 10.1111/jfd.14152 |