Breaking the Selectivity Barrier of Single-Atom Nanozymes Through Out-of-Plane Ligand Coordination

© 2025 The Author(s). Advanced Materials published by Wiley‐VCH GmbH.

Détails bibliographiques
Publié dans:Advanced materials (Deerfield Beach, Fla.). - 1998. - 37(2025), 38 vom: 04. Sept., Seite e2506480
Auteur principal: Park, Seonhye (Auteur)
Autres auteurs: Shim, Kyu In, Nguyen, Phuong Thy, Choi, Daeeun, Kim, Seongbeen, Yi, Seung Yeop, Kim, Moon Il, Han, Jeong Woo, Lee, Jinwoo
Format: Article en ligne
Langue:English
Publié: 2025
Accès à la collection:Advanced materials (Deerfield Beach, Fla.)
Sujets:Journal Article biosensing out‐of‐plane ligand coordination peroxidase‐like activity point‐of‐care testing single‐atom nanozyme Ligands Hydrogen Peroxide BBX060AN9V Ruthenium plus... 7UI0TKC3U5 Chlorine 4R7X1O2820 Catalase EC 1.11.1.6 Peroxidase EC 1.11.1.7
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520 |a Peroxidase (POD)-like nanozymes have emerged as effective alternatives to natural enzymes owing to their stability and cost-effectiveness in biosensors. In particular, single-atom nanozymes (SAzymes) featuring Fe-N4 active sites have attracted significant attention for their high catalytic performance. However, their 2D exposed active sites result in limited reaction selectivity and strong pH dependence, restricting their functionality under neutral conditions. This study introduces Ru-centered SAzymes coordinated out-of-plane with chlorine ligands (RuNC_Cl), achieving monofunctional POD-like activity. RuNC_Cl exhibited remarkable POD-like activity, which is 38-fold greater than its catalase (CAT)-like activity, indicating strong suppression of the competing CAT-like reaction. Density functional theory calculations and Bader charge analysis of RuNC_Cl reveal that repulsive forces preventing secondary H2O2 adsorption contribute to an increased energy barrier for the CAT-like reaction. This selective POD-like activity enables the precise detection of multiple biomarkers through a one-pot cascade reaction under near-neutral conditions. This advancement paves the way for the precise regulation of reaction pathways, enhancing the practicality of nanozymes for biosensing and related applications 
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700 1 |a Shim, Kyu In  |e verfasserin  |4 aut 
700 1 |a Nguyen, Phuong Thy  |e verfasserin  |4 aut 
700 1 |a Choi, Daeeun  |e verfasserin  |4 aut 
700 1 |a Kim, Seongbeen  |e verfasserin  |4 aut 
700 1 |a Yi, Seung Yeop  |e verfasserin  |4 aut 
700 1 |a Kim, Moon Il  |e verfasserin  |4 aut 
700 1 |a Han, Jeong Woo  |e verfasserin  |4 aut 
700 1 |a Lee, Jinwoo  |e verfasserin  |4 aut 
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773 1 8 |g volume:37  |g year:2025  |g number:38  |g day:04  |g month:09  |g pages:e2506480 
856 4 0 |u http://dx.doi.org/10.1002/adma.202506480  |3 Volltext 
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