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20250920232136.0 |
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250920s2025 xx |||||o 00| ||eng c |
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|a 10.1111/nph.70473
|2 doi
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|a pubmed25n1574.xml
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|a (DE-627)NLM392740656
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|a (NLM)40836608
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Huang, Xiaoyu
|e verfasserin
|4 aut
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| 245 |
1 |
4 |
|a The m6A writer complex function in main stem apex development by promoting mRNA stability of GIF3, HDA6, and KNAT2/6 in Gossypium hirsutum
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| 264 |
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|c 2025
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 18.09.2025
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|a Date Revised 18.09.2025
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2025 The Author(s). New Phytologist © 2025 New Phytologist Foundation.
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|a N6-methyladenosine (m6A) is the most abundant internal posttranscriptional modification of eukaryotic messenger RNA (mRNA), which can be involved in the regulation of mRNA metabolism and processing. Although some progress has been made in understanding the methylation mechanism of mRNA m6A in plants, our knowledge and comprehension of this process in cotton is still significantly limited. In this study, we identified the cotton m6A writers, namely GhMTA-A/D, GhMTB-A/D, and GhFIP37-A/D. These writers are localized within the nucleus. Notably, GhMTA-D has been found to interact with both GhMTB-A and GhFIP37-A. The knockdown of each cotton m6A writer gene, achieved through virus-induced gene silencing, resulted in decreased mRNA m6A levels in cotton main stem apex (MSA). This led to a thicker shoot apical meristem, the formation of an abnormal MSA, and dwarf plants. MeRIP-seq and RNA-seq analyses showed that m6A writers mediate m6A modification and transcripts of mRNAs in cotton MSA by recognizing the motif GGACH and/or GAAGM. Furthermore, m6A writers install m6A in GhGIF3, GhHDA6, and GhKNAT2/6 transcripts, thereby affecting their mRNA stability and regulating MSA development. In summary, our research elucidates a novel mechanism for m6A posttranscriptional regulation and offers valuable insights into the roles of m6A writers in controlling MSA development
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|a Journal Article
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|a cotton
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|a m6A
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|a mRNA stability
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4 |
|a main stem apex development
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| 650 |
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4 |
|a writer complex
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| 650 |
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|a RNA, Messenger
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 650 |
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|a N-methyladenosine
|2 NLM
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| 650 |
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7 |
|a CLE6G00625
|2 NLM
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| 650 |
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|a Adenosine
|2 NLM
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| 650 |
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|a K72T3FS567
|2 NLM
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| 700 |
1 |
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|a Zhang, Hui
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liao, Huiping
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Tao, Miao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Xiaoqian
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liu, Yingshan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Xinyue
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Jingbo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Dengdi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xu, Wenliang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhao, Huixin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Huang, Gengqing
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g 248(2025), 2 vom: 01. Sept., Seite 721-740
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnas
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| 773 |
1 |
8 |
|g volume:248
|g year:2025
|g number:2
|g day:01
|g month:09
|g pages:721-740
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| 856 |
4 |
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|u http://dx.doi.org/10.1111/nph.70473
|3 Volltext
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|a GBV_ILN_350
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|a AR
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|d 248
|j 2025
|e 2
|b 01
|c 09
|h 721-740
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