Drought-activated BZR1 reprograms flavonoid metabolism via transcriptional cascades to amplify baicalin biosynthesis in Scutellaria baicalensis

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 227(2025) vom: 01. Sept., Seite 110196
1. Verfasser:	Li, Xian (VerfasserIn)
Weitere Verfasser:	Chen, Chong, Cao, Bo, Feng, Shan, Zhang, Yali, Bin, Tiantian, Zhou, Xiaofang, Liu, Lianjin, Hu, Suying, Zheng, Bowen, Li, Guishuang, Bai, Chengke
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2025
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Journal Article Drought resilience Flavonoid biosynthesis Glycoside-aglycone ratio Growth-defense trade-off Metabolic reprogramming SbBZR1 Flavonoids baicalin 347Q89U4M5 mehr... Transcription Factors Arabidopsis Proteins Plant Proteins BZR1 protein, Arabidopsis DNA-Binding Proteins


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245	1	0	\|a Drought-activated BZR1 reprograms flavonoid metabolism via transcriptional cascades to amplify baicalin biosynthesis in Scutellaria baicalensis
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520			\|a Environmental stress triggers a dual response in medicinal plants by inhibiting growth while enhancing secondary metabolite production. However, the regulatory mechanism of this trade-off remains unclear. Here, we identified SbBZR1 as a brassinosteroid (BR)-responsive transcription factor that orchestrates drought resilience and baicalin biosynthesis in Scutellaria baicalensis through integrated multi-omics and metabolic flux analysis. In general, drought stress upregulated BR signaling and SbBZR1 expression. Transgenic Arabidopsis expressing SbBZR1 and its hyperactive mutant (Sbbzr1-1D) exhibited enhanced drought tolerance: 57 % greater biomass, 43 % increased root density, and 62 % reduced lipid peroxidation via antioxidant gene activation (AtDHAR1, AtCAT2, AtSOD1). In S. baicalensis hairy roots, SbBZR1 overexpression increased biomass (68 %) and anthocyanins (2.3-fold), while upregulating flavonoid genes (SbPAL, SbCHS, SbCHI). Under gradient drought, SbBZR1 reprogrammed metabolism dose-dependently: mild stress (1 % PEG) elevated both glycosides (baicalin +128 %) and aglycones (baicalein +196 %), whereas Sbbzr1-1D prioritized glycoside production (3.42-fold baicalin; glycoside-aglycone ratio 15.02 vs. WT 3.93). Mechanistically, Sbbzr1-1D amplified phenylpropanoid flux via SbPAL and SbF8H (4.1-6.3 fold) while suppressing deglycosylation (β-glucuronidase, SbGUS 72 % down-expression). Exogenous epibrassinolide (eBL) treatment confirmed SbBZR1 amplifies BR sensitivity, boosting baicalin yields 2.1-3.8 fold. The BR-SbBZR1 axis dynamically coordinates drought-induced transcriptional bursts (SbFNSII-2, SbF6H) with flavonoid accrual, while differentially regulating glycosylation (SbUBGAT up-expression) and deglycosylation (SbGUS down-expression). Collectively, these results first reveal the transcriptional logic of stress-driven phytochemical enhancement and provide valuable references for engineering drought-resistant crops through precision metabolic regulation
650		4	\|a Journal Article
650		4	\|a Drought resilience
650		4	\|a Flavonoid biosynthesis
650		4	\|a Glycoside-aglycone ratio
650		4	\|a Growth-defense trade-off
650		4	\|a Metabolic reprogramming
650		4	\|a SbBZR1
650		7	\|a Flavonoids \|2 NLM
650		7	\|a baicalin \|2 NLM
650		7	\|a 347Q89U4M5 \|2 NLM
650		7	\|a Transcription Factors \|2 NLM
650		7	\|a Arabidopsis Proteins \|2 NLM
650		7	\|a Plant Proteins \|2 NLM
650		7	\|a BZR1 protein, Arabidopsis \|2 NLM
650		7	\|a DNA-Binding Proteins \|2 NLM
700	1		\|a Chen, Chong \|e verfasserin \|4 aut
700	1		\|a Cao, Bo \|e verfasserin \|4 aut
700	1		\|a Feng, Shan \|e verfasserin \|4 aut
700	1		\|a Zhang, Yali \|e verfasserin \|4 aut
700	1		\|a Bin, Tiantian \|e verfasserin \|4 aut
700	1		\|a Zhou, Xiaofang \|e verfasserin \|4 aut
700	1		\|a Liu, Lianjin \|e verfasserin \|4 aut
700	1		\|a Hu, Suying \|e verfasserin \|4 aut
700	1		\|a Zheng, Bowen \|e verfasserin \|4 aut
700	1		\|a Li, Guishuang \|e verfasserin \|4 aut
700	1		\|a Bai, Chengke \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Plant physiology and biochemistry : PPB \|d 1991 \|g 227(2025) vom: 01. Sept., Seite 110196 \|w (DE-627)NLM098178261 \|x 1873-2690 \|7 nnas
773	1	8	\|g volume:227 \|g year:2025 \|g day:01 \|g month:09 \|g pages:110196
856	4	0	\|u http://dx.doi.org/10.1016/j.plaphy.2025.110196 \|3 Volltext
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