CbuERF114, an ethylene responsive factor, regulates floral transition in Catalpa bungei
Copyright © 2025 Elsevier Masson SAS. All rights reserved.
| Publié dans: | Plant physiology and biochemistry : PPB. - 1991. - 227(2025) vom: 01. Sept., Seite 110176 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , , , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2025
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| Accès à la collection: | Plant physiology and biochemistry : PPB |
| Sujets: | Journal Article AP2/ERF Catalpa bungei CbuERF114 Floral transition Flower quantity Regulation of flowering time |
| Résumé: | Copyright © 2025 Elsevier Masson SAS. All rights reserved. Understanding the flowering of woody plants is crucial for controlling flowering time and shortening breeding cycles, yet the mechanisms are largely unknown. APETALA2/ethylene response factor (AP2/ERF) transcription factors are essential in floral transition and development. We report an early-flowering (EF) variety of Catalpa bungei that blooms in its planting year, whereas normal-flowering (NF) requires 5-7 years. This study identified 150 CbuAP2/ERF genes in the C. bungei genome, categorized into 4 subfamilies and 17 groups. Expression analysis showed AP2/ERF genes mainly activated during the vegetative to mixed buds transition and predominantly expressed in the stem. To explore CbuERF roles in floral transition, six genes (CbuERF3B/060/071/RAP2-3/114/003-4) induced by floral induction were selected. CbuERF114 expression differed significantly during the reproductive transition between NF and EF. Subcellular localization and transcriptional activation analysis indicated that CbuERF114 is a nuclear protein with a C-terminal transcriptional activation domain. Overexpressing CbuERF114 in Arabidopsis (OE-CbuERF114) delayed bolting and flowering by about 4 days but extended the flowering period, increasing the maximum flowering growth rate by 54.25% and the number of flowers by 58.00%. CbuERF114 inhibited flowering onset but enhanced flowering rate and quantity. These findings advance our understanding of CbuAP2/ERFs in phylogeny, structure, expression, and function, highlighting their importance in the floral transition of woody plants |
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| Description: | Date Revised 16.09.2025 published: Print-Electronic Citation Status In-Process |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2025.110176 |