Co-overexpression of ZlRc and ZlRd increases flavonoid content, antioxidant activity, and inhibitory effects on against α-glucosidase, α-amylase, pancreatic lipase, and tyrosinase without affecting rice agronomic traits or yield
Copyright © 2025 Elsevier Masson SAS. All rights reserved.
| Publié dans: | Plant physiology and biochemistry : PPB. - 1991. - 227(2025) vom: 12. Sept., Seite 110137 |
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| Auteur principal: | |
| Autres auteurs: | , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2025
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| Accès à la collection: | Plant physiology and biochemistry : PPB |
| Sujets: | Journal Article Agronomic traits Antioxidant activity Co-overexpression Flavonoid Transcriptome |
| Résumé: | Copyright © 2025 Elsevier Masson SAS. All rights reserved. Pigmented rice (e.g., black or purple rice), rich in flavonoids and other active compounds, has lower yields than non-pigmented rice. The study aimed to breed pigmented rice with stable yields. To this end, we created transgenic rice by inserting the ZlRc and ZlRd genes from Chinese wild rice (Zizania latifolia) into rice (Oryza sativa) and investigated the resulting yield and flavonoid content. Rc encodes a transcription factor that regulates flavonoid biosynthesis, while dihydroflavonol 4-reductase (DFR/Rd) is an important flavonoid biosynthetic enzyme. The dark brown colour of the pericarp rice was attributed to the co-overexpression of ZlRc and ZlRd (ZlRcRd), which did not influence the agronomic traits or yield. No significant difference was observed in the chlorophyll content or photosynthetic gas exchange parameters between the ZlRcRd and wild-type (WT) rice. The flavonoid content in the ZlRcRd rice seeds was significantly enhanced, and so were antioxidant activity and inhibitory effects on α-glucosidase, α-amylase, pancreatic lipase, and tyrosinase. Compared with WT rice, 1080 genes and 82 flavonoids were upregulated in the ZlRcRd rice. The expression of key genes involved in flavonoid biosynthesis (CHS, F3'H, and F3'5'H) and the activities of related key enzymes were significantly higher in ZlRcRd than WT rice. This study proposes new germplasm resources and technical approaches for breeding pigmented rice with stable yields and high flavonoid content, which will address the challenge of low yields of pigmented rice and increase farmers' enthusiasm for planting pigmented rice |
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| Description: | Date Revised 16.09.2025 published: Print-Electronic Citation Status In-Process |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2025.110137 |