Spray Modification of Amino-Functionalized g-C3N4 Nanosheets on Screen-Printed Carbon Electrodes for the Electrochemiluminescence Immunoassay of SARS-CoV-2 N Protein Based on Coreactant Release from Polymersome Tags
A signal-on electrochemiluminescence (ECL) immunoassay was developed for the detection of the SARS-CoV-2 N protein using coreactant-encapsulated polymersomes as tags and a graphitic carbon nitride (g-C3N4) nanosheet-modified screen-printed carbon electrode (SPCE) as the working electrode. Poly(ether...
| Publié dans: | Langmuir : the ACS journal of surfaces and colloids. - 1985. - 41(2025), 37 vom: 23. Sept., Seite 25556-25564 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2025
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| Accès à la collection: | Langmuir : the ACS journal of surfaces and colloids |
| Sujets: | Journal Article graphitic carbon nitride Graphite 7782-42-5 Carbon 7440-44-0 Nitrogen Compounds Coronavirus Nucleocapsid Proteins nucleocapsid phosphoprotein, SARS-CoV-2 Nitriles plus... |
| Résumé: | A signal-on electrochemiluminescence (ECL) immunoassay was developed for the detection of the SARS-CoV-2 N protein using coreactant-encapsulated polymersomes as tags and a graphitic carbon nitride (g-C3N4) nanosheet-modified screen-printed carbon electrode (SPCE) as the working electrode. Poly(ether imide) (PEI)-modified g-C3N4 nanosheets were prepared through ultrasonic exfoliation followed by a supramolecular covalent scaffold strategy. Spray coating, a scalable deposition technique, was employed to modify the SPCE surface with g-C3N4 nanosheets. After thermal curing, the nanosheets adhered firmly to the electrode via binders in the carbon ink, yielding a stable modified SPCE. Immunosensors were constructed by immobilizing capture antibodies on the g-C3N4-modified SPCE. Potassium persulfate, (K2S2O8), the coreactant of g-C3N4, was encapsulated in polymersomes to prepare ECL tags, and probes were fabricated by conjugating SARS-CoV-2 N protein binding aptamers to these polymersomes. Upon recognition of the target protein and sandwich complexes formed on the immunosensor, the subsequent addition of Triton X-100 disrupted the polymersomes, releasing large amounts of K2S2O8, which markedly enhanced the ECL mission of g-C3N4 nanosheets. This amplification enabled highly sensitive detection of the SARS-CoV-2 N protein. These findings demonstrate that controlled release of encapsulated coreactants from polymersome tags is an effective strategy to trigger g-C3N4-based ECL emission for sensitive viral protein detection |
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| Description: | Date Completed 23.09.2025 Date Revised 23.09.2025 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1520-5827 |
| DOI: | 10.1021/acs.langmuir.5c03372 |