The phytochrome-interacting factor CsPIF3a positively regulates chlorophyll biosynthesis in tea plants (Camellia sinensis)

Copyright © 2025. Published by Elsevier Masson SAS.

Détails bibliographiques
Publié dans:Plant physiology and biochemistry : PPB. - 1991. - 229(2025), Pt B vom: 25. Aug., Seite 110419
Auteur principal: Zhang, Xiangna (Auteur)
Autres auteurs: Tong, Jiewen, Wen, Beibei, Li, Saijun, Xiong, Ligui, Li, Juan, Sun, Mufang, Guo, Guiyi, Huang, Jian-An
Format: Article en ligne
Langue:English
Publié: 2025
Accès à la collection:Plant physiology and biochemistry : PPB
Sujets:Journal Article Camellia sinensis Chlorophyll biosynthesis PIF transcription factors Transcriptional regulation
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520 |a Chlorophyll corresponds to the green pigments in leaves and forms the color of leaves together with carotenoids, which also plays a crucial role in growth and yield in tea plants. Although genes involved in chlorophyll biosynthesis have been widely reported, the regulatory mechanism of transcription factors on chlorophyll biosynthesis remains to be further elucidated in tea plants. Here, we found chlorophyll content was strongly correlated with the expression of chlorophyll biosynthetic genes CsHEMA and CsPOR in tea leaves with different leaf colors. Additionally, a phytochrome-interacting factor CsPIF3a was identified, and its expression was positively correlated with chlorophyll content and the expression of CsHEMA and CsPOR. CsPIF3a is a nuclear-localized protein with self-activating transcriptional property. Furthermore, the electrophoretic mobility shift assay illustrated that CsPIF3a directly binds to the promoters of CsHEMA and CsPOR to activate their transcription. A dual-luciferase assay confirmed that CsPIF3a could activate the expression of CsHEMA and CsPOR. Overexpression of CsPIF3a in Arabidopsis thaliana resulted in a significant increase in chlorophyll accumulation and the expression of biosynthesis-related genes. Collectively, our study demonstrates that CsPIF3a acts as a transcriptional activator to regulate chlorophyll biosynthesis in tea plants. These findings provide novel insights into the transcriptional regulatory mechanism of PIFs associated with chlorophyll biosynthesis, which might be helpful to breed leaf color-specific tea cultivars 
650 4 |a Journal Article 
650 4 |a Camellia sinensis 
650 4 |a Chlorophyll biosynthesis 
650 4 |a PIF transcription factors 
650 4 |a Transcriptional regulation 
700 1 |a Tong, Jiewen  |e verfasserin  |4 aut 
700 1 |a Wen, Beibei  |e verfasserin  |4 aut 
700 1 |a Li, Saijun  |e verfasserin  |4 aut 
700 1 |a Xiong, Ligui  |e verfasserin  |4 aut 
700 1 |a Li, Juan  |e verfasserin  |4 aut 
700 1 |a Sun, Mufang  |e verfasserin  |4 aut 
700 1 |a Guo, Guiyi  |e verfasserin  |4 aut 
700 1 |a Huang, Jian-An  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant physiology and biochemistry : PPB  |d 1991  |g 229(2025), Pt B vom: 25. Aug., Seite 110419  |w (DE-627)NLM098178261  |x 1873-2690  |7 nnas 
773 1 8 |g volume:229  |g year:2025  |g number:Pt B  |g day:25  |g month:08  |g pages:110419 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2025.110419  |3 Volltext 
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