RXLR effectors from Phytophthora infestans suppress host cell death and promote virulence via nuclear localization in Nicotiana benthamiana

Copyright © 2025 Elsevier B.V. All rights reserved.

Détails bibliographiques
Publié dans:Plant science : an international journal of experimental plant biology. - 1985. - 362(2025) vom: 23. Aug., Seite 112730
Auteur principal: Park, SangA (Auteur)
Autres auteurs: Kim, Taewon, Lee, Soeui, Seo, Ye-Eun, In, Solhee, Choi, Doil, Lee, Joo Hyun
Format: Article en ligne
Langue:English
Publié: 2025
Accès à la collection:Plant science : an international journal of experimental plant biology
Sujets:Journal Article Cell death Cell death inducer (CDI) Cell death suppressor (CDS) Hemibiotroph Phytophthora infestans RXLR effectors
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520 |a Phytophthora infestans, a destructive hemibiotrophic pathogen, relies on effector proteins to modulate host immunity and facilitate infection. However, the molecular mechanisms by which these effectors suppress host cell death during the biotrophic phase remain poorly understood. In this study, we identified three RXLR effectors-designated as Cell Death Suppressors (CDS1: Pi02860, CDS2: Pi04089, and CDS5: Pi06099)-that strongly suppress effector-triggered cell death in Nicotiana benthamiana. These CDSs are highly expressed during the early biotrophic phase and inhibit cell death triggered by diverse elicitors, including INF1, NPP1.1, Rpiblb2/Avrblb2, and Avr3a/R3a. Subcellular localization analysis revealed that the nuclear targeting is essential for their cell death suppressive activity. Functional assays demonstrated that CDS1 significantly enhances P. infestans virulence, while CDS2 and CDS5 contribute synergistically when co-expressed. Moreover, homologs of CDS1 from other Phytophthora species exhibited similar suppressive activity, despite lacking obvious sequence or structural features predictive of function. Our findings suggest that P. infestans utilizes a coordinated set of nuclear-targeting effectors to suppress host immunity during early colonization, offering new insights into pathogen strategies for immune evasion 
650 4 |a Journal Article 
650 4 |a Cell death 
650 4 |a Cell death inducer (CDI) 
650 4 |a Cell death suppressor (CDS) 
650 4 |a Hemibiotroph 
650 4 |a Phytophthora infestans 
650 4 |a RXLR effectors 
700 1 |a Kim, Taewon  |e verfasserin  |4 aut 
700 1 |a Lee, Soeui  |e verfasserin  |4 aut 
700 1 |a Seo, Ye-Eun  |e verfasserin  |4 aut 
700 1 |a In, Solhee  |e verfasserin  |4 aut 
700 1 |a Choi, Doil  |e verfasserin  |4 aut 
700 1 |a Lee, Joo Hyun  |e verfasserin  |4 aut 
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