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250903s2025 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2025.110407
|2 doi
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|a eng
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|a Xiang, Xiaoe
|e verfasserin
|4 aut
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|a Manipulation of conserved BnFUSCA3s gene instead of using its allelic variation increases the seed oil content of rapeseed
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|c 2025
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|a Text
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|a ƒaComputermedien
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|a Date Revised 22.08.2025
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|a published: Print-Electronic
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|a Citation Status Publisher
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|a Copyright © 2025 Elsevier Masson SAS. All rights reserved.
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|a FUSCA3 (FUS3) is a B3 domain transcription factor that plays a vital role in regulating embryo development. Despite its importance, the exact mechanism by which FUS3 regulates seed oil biosynthesis remains unclear. In our study, we identified four BnFUS3 members in Brassica napus, sharing closely conserved gene structure and protein sequences. GUS staining revealed that all four BnFUS3 genes were expressed in young embryos located in the nucleus. When BnFUS3-1 was overexpressed with XVE, an estrogen receptor-based chemical-inducible system for screening transgenic plants, Arabidopsis seedlings exhibited growth retardation or even death, accompanied by an increase in oil content. However, analysis of re-sequenced SNPs of two varieties showed that the four BnFUS3s had no significant correlation with oil content and storage protein. Although no transgenic plants had been screened using the 35S promoter-driven BnFUS3-1, when BnFUS3 genes were driven by a seed-specific promoter D6, the oil content of transgenic B. napus seeds increased by 6.9-21.5 % without affecting agronomic traits. In these transgenic seeds, numerous genes related to photosynthesis, fatty acid biosynthesis, and glycolysis were up-regulated. This led to an increase in starch accumulation, while sucrose and glucose decreased, indicating a higher carbon flux towards fatty acid biosynthesis. Our findings highlight the crucial role of BnFUS3s in regulating oil synthesis during rapeseed maturation. While BnFUS3s do not exhibit significant allelic variation that can be exploited in traditional breeding methods to develop high-oil content varieties, transgenic manipulation of BnFUS3s shows promise in enhancing oil production and quality through genetic improvement
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|a Journal Article
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|a BnFUS3
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|a Brassica napus
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|a Fatty acid biosynthesis
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| 650 |
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|a Rapeseed oil
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| 650 |
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|a Seed development
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| 700 |
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|a Qi, Xiao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Heidari, Bahram
|e verfasserin
|4 aut
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| 700 |
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|a Wang, Xingchun
|e verfasserin
|4 aut
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| 700 |
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|a Tan, Helin
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 229(2025), Pt A vom: 19. Aug., Seite 110407
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|x 1873-2690
|7 nnas
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|g volume:229
|g year:2025
|g number:Pt A
|g day:19
|g month:08
|g pages:110407
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|u http://dx.doi.org/10.1016/j.plaphy.2025.110407
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