Comparison of Three Feline Crossmatch Methods-Tube, Gel Column, and Immunochromatographic Strip

© 2025 The Author(s). Veterinary Clinical Pathology published by Wiley Periodicals LLC on behalf of American Society for Veterinary Clinical Pathology.

Détails bibliographiques
Publié dans:Veterinary clinical pathology. - 1975. - 54(2025), 3 vom: 22. Sept., Seite 230-238
Auteur principal: DiCiccio, Victoria K (Auteur)
Autres auteurs: Hess, Rebecka S, Weinstein, Nicole M, Fromm, Samantha, Gonzalez, Ruth, Marryott, Kimberly, Callan, Mary Beth
Format: Article en ligne
Langue:English
Publié: 2025
Accès à la collection:Veterinary clinical pathology
Sujets:Journal Article Comparative Study RBC incompatibility alloantibody antiglobulin transfusion
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245 1 0 |a Comparison of Three Feline Crossmatch Methods-Tube, Gel Column, and Immunochromatographic Strip 
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500 |a Date Revised 20.09.2025 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a © 2025 The Author(s). Veterinary Clinical Pathology published by Wiley Periodicals LLC on behalf of American Society for Veterinary Clinical Pathology. 
520 |a BACKGROUND: A pre-transfusion blood crossmatch is recommended to ensure RBC compatibility for previously transfused and transfusion-naïve cats 
520 |a OBJECTIVES: To compare 3 crossmatch methods and anti-feline globulin (AFG) enhancement for determining RBC compatibility 
520 |a METHODS: Prospective study. Major crossmatches were performed using serum from 68 "recipient" cats and RBCs from 44 "donor" cats. Crossmatch methods evaluated include laboratory tube and gel column without and with AFG enhancement and an in-clinic AFG-enhanced immunochromatographic strip 
520 |a RESULTS: Tube and gel crossmatches were performed for 211 and strip crossmatches for 133 recipient-donor pairs. RBC incompatibilities were noted in at least 1 crossmatch method for 123 recipient-donor pairs. For determination of the degree of incompatibility, there was a correlation between standard and AFG-enhanced crossmatches for both tube and gel (p < 0.001), standard tube and gel (p = 0.002), and AFG-enhanced tube and gel (p < 0.001). Twelve of 46 and 22 of 113 recipient-donor pairs deemed incompatible with tube and gel, respectively, had RBC agglutination noted only with the addition of AFG. RBC incompatibilities were noted on 15 strips, 4 of which were type A-B mismatched. Odds of detecting RBC incompatibility using strip were 2.9 (p = 0.001) and 6.1 (p < 0.001) times greater with each unit increase in the degree of incompatibility detected by standard and AFG-enhanced tube, respectively 
520 |a CONCLUSIONS: The clinical relevance of any observed RBC incompatibilities, other than known A-B mismatches, is unknown. While the strip crossmatch is simple to perform and interpret, it might not be sufficiently sensitive to detect weaker RBC incompatibilities 
650 4 |a Journal Article 
650 4 |a Comparative Study 
650 4 |a RBC incompatibility 
650 4 |a alloantibody 
650 4 |a antiglobulin 
650 4 |a transfusion 
700 1 |a Hess, Rebecka S  |e verfasserin  |4 aut 
700 1 |a Weinstein, Nicole M  |e verfasserin  |4 aut 
700 1 |a Fromm, Samantha  |e verfasserin  |4 aut 
700 1 |a Gonzalez, Ruth  |e verfasserin  |4 aut 
700 1 |a Marryott, Kimberly  |e verfasserin  |4 aut 
700 1 |a Callan, Mary Beth  |e verfasserin  |4 aut 
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773 1 8 |g volume:54  |g year:2025  |g number:3  |g day:22  |g month:09  |g pages:230-238 
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