Characterization of MADS-box genes in Chinese cherry and overexpression of CpMADS2 promoting early flowering in transgenic Arabidopsis
Copyright © 2025 Elsevier GmbH. All rights reserved.
| Publié dans: | Journal of plant physiology. - 1979. - 312(2025) vom: 28. Sept., Seite 154583 |
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| Auteur principal: | |
| Autres auteurs: | , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2025
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| Accès à la collection: | Journal of plant physiology |
| Sujets: | Journal Article Abnormal flowering Chinese cherry Flowering time MADS-box MADS Domain Proteins Plant Proteins |
| Résumé: | Copyright © 2025 Elsevier GmbH. All rights reserved. The Chinese cherry (Cerasus pseudocerasus Lindl.) cv. 'Manaohong', a distinctive cultivar indigenous to Guizhou Province, China, possesses significant nutritional and economic value. In our previous studies, we observed an abnormal flowering phenomenon in the 'Manaohong' cherry. The MADS-box gene family plays a pivotal role in regulating flowering time and the floral organs development in plants. In this study, we identified 51 CpMADS genes. Transcriptome analysis revealed that the PI-like gene CpMADS2 was significantly differentially expressed between normal flowers (NF) and abnormal flowers (AF) in Chinese cherry. To further investigate the functional role of CpMADS2, it was cloned from the Chinese cherry. Overexpression of CpMADS2 in Arabidopsis thaliana exhibited earlier flowering compared to wild-type. Yeast two-hybrid (Y2H) assay and luciferase complementation assay (LCA) confirmed that CpMADS2 interacts with the CpFT, suggesting that CpMADS2 may promotes flowering by interacting with CpFT. This study provides a theoretical foundation for further research on the role of CpMADS2 in regulating abnormal flowering in Chinese cherry |
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| Description: | Date Completed 31.08.2025 Date Revised 31.08.2025 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1618-1328 |
| DOI: | 10.1016/j.jplph.2025.154583 |