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250817s2025 xx |||||o 00| ||eng c |
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|a 10.1080/07366205.2025.2542012
|2 doi
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|a eng
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| 100 |
1 |
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|a Toleco, M Rey
|e verfasserin
|4 aut
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| 245 |
1 |
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|a A simple and universal quasi-modular cloning system using NEBuilder® HiFi DNA assembly kit
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|c 2025
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 05.09.2025
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|a Date Revised 05.09.2025
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Modular cloning has transformed synthetic biology by enabling rapid assembly of standardized DNA parts, but its effectiveness is often limited to well-characterised model organisms. Here, we expand the utility of the NEBuilder® HiFi DNA Assembly Kit (New England Biolabs) into a quasi-modular system that uses single-stranded oligonucleotide bridges to assemble reusable DNA fragments without the need for restriction enzymes. Using the anaerobic gut eukaryote Blastocystis ST7-B as a test case, we constructed and reassembled vectors with diverse promoter-terminator combinations. These results underscore the versatility of the method and its potential to accelerate genetic tool development in non-model biological systems
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| 650 |
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4 |
|a Journal Article
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|a Blastocystis
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|a NEBuilder® HiFi DNA assembly
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| 650 |
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|a Non-model organisms
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|a genetic tool development
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|a modular cloning
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| 650 |
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|a DNA
|2 NLM
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| 650 |
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|a 9007-49-2
|2 NLM
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| 700 |
1 |
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|a van der Giezen, Mark
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t BioTechniques
|d 1991
|g 77(2025), 5-6 vom: 16. Mai, Seite 257-262
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnas
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| 773 |
1 |
8 |
|g volume:77
|g year:2025
|g number:5-6
|g day:16
|g month:05
|g pages:257-262
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|u http://dx.doi.org/10.1080/07366205.2025.2542012
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