SmGATA15 interacts with SmMYC2 to positively regulate cold stress tolerance by modulating chlorophyll accumulation in Solanum melongena
Copyright © 2025 Elsevier Masson SAS. All rights reserved.
| Publié dans: | Plant physiology and biochemistry : PPB. - 1991. - 229(2025), Pt A vom: 11. Aug., Seite 110366 |
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| Auteur principal: | |
| Autres auteurs: | , , , , , , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2025
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| Accès à la collection: | Plant physiology and biochemistry : PPB |
| Sujets: | Journal Article Chlorophyll metabolism Cold tolerance SmGATA15 SmMYC2 Solanum melongena |
| Résumé: | Copyright © 2025 Elsevier Masson SAS. All rights reserved. Chlorophyll homeostasis plays a pivotal role in plant adaptation to cold stress by maintaining photosynthetic efficiency and reducing oxidative damage. However, mechanisms regulating chlorophyll biosynthesis in eggplant (Solanum melongena L.) under cold stress are imperfectly known remain unclear. We measured chlorophyll contents in eggplant leaves were increased significantly (p < 0.05) under cold stress and isolated the core regulatory factor SmGATA15 for chlorophyll metabolism through transcriptomics and gene cloning. The experiments of SmGATA15 heterologous overexpression in Arabidopsis thaliana indicated that it plays important roles in chlorophyll biosynthesis and enhances peroxidase activity and the gene expression levels of genes within the ICE-CBF-COR (inducer of CBF expression-C-repeat binding factor-cold-responsive) regulatory network. Four key chlorophyll structural genes (e.g., SmHEMA, SmCHLG, SmPORA, and SmPOA) were identified through transcriptome and promoter elements prediction analysis, and SmGATA15 bond to promoters of genes and regulates its activity. SmMYC2 interacted with SmGATA15, promoting SmGATA15-mediated transcriptional activation. Heterologous overexpression of SmMYC2 exerted a positive regulatory effect on chlorophyll content and cold tolerance under cold stress. In conclusion, these results improved our understanding of the molecular mechanisms of SmGATA15 enhances eggplant cold tolerance by interacting with SmMYC2 to increase chlorophyll content, peroxidase activity and influence the expression of ICE-CBF-COR related genes |
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| Description: | Date Revised 16.08.2025 published: Print-Electronic Citation Status Publisher |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2025.110366 |