Development of a Real-Time PCR Assay to Detect the Kiwifruit Pathogen Pectobacterium actinidiae
Pectobacterium actinidiae is a significant kiwifruit canker pathogen reported from kiwifruit-growing areas in Asia and South America. To improve the diagnostic capability for this bacterium, a probe-based real-time PCR assay was developed for sensitive and fast detection of P. actinidiae. The highly...
| Veröffentlicht in: | Plant disease. - 1997. - 109(2025), 8 vom: 13. Aug., Seite 1683-1689 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2025
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| Zugriff auf das übergeordnete Werk: | Plant disease |
| Schlagworte: | Journal Article Actinidia chinensis Pectobacterium carotovorum pv. actinidiae bacterial canker biosecurity fruit pathogen detection prokaryotes summer canker |
| Zusammenfassung: | Pectobacterium actinidiae is a significant kiwifruit canker pathogen reported from kiwifruit-growing areas in Asia and South America. To improve the diagnostic capability for this bacterium, a probe-based real-time PCR assay was developed for sensitive and fast detection of P. actinidiae. The highly specific assay targets the meta-pathway phenol degradation protein-like locus, unique to P. actinidiae. Validation showed that the assay is highly sensitive, detecting the pathogen down to 0.1 pg of pure genomic DNA or 10 to 50 colony-forming units in plant tissue matrix. To assess the competency of the tested DNA samples and avoid false-negative results, the assay was duplexed with plant internal control. This new method provides a valuable tool for specific and sensitive testing of imported and exported plant material to ensure freedom from P. actinidiae and prevent the spread of this pathogen into new areas.[Formula: see text] Copyright © 2025 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license |
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| Beschreibung: | Date Completed 01.09.2025 Date Revised 01.09.2025 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 0191-2917 |
| DOI: | 10.1094/PDIS-05-24-1092-RE |