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20250812232105.0 |
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250812s2025 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2025.112653
|2 doi
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|a pubmed25n1528.xml
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|a (DE-627)NLM390986720
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|a (NLM)40633585
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|a (PII)S0168-9452(25)00271-7
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Hernández-Esquivel, Alma Alejandra
|e verfasserin
|4 aut
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|a Phospholipase D is required for the activation of target of rapamycin (TOR) signaling in Arabidopsis thaliana by lipopolysaccharides from Azospirillum baldaniorum Sp245
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|c 2025
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 12.08.2025
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|a Date Revised 12.08.2025
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2025 Elsevier B.V. All rights reserved.
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|a Phospholipase D (PLD) metabolize phospholipids in cell membranes to produce phosphatidic acid (PA), a second messenger involved in activating signaling pathways associated with developmental processes and responses to various environmental stresses. This study investigated the effect of Azospirillum baldaniorum lipopolysaccharides (LPS) on PLD activity and its relationship with the Target of Rapamycin (TOR) signaling pathway during the growth promotion of Arabidopsis thaliana. Treatment with LPS (100 µg/mL) increased PLD activity with 5 min, with a more pronounced effect observed at 30 min. PA content also increased in parallel with PLD activity, reaching its peak at 30 min. LPS failed to promote growth in the xipot1 mutant, which is defective in phosphocholine biosynthesis, a precursor of phosphatidylcoline, the substrate of PLD, and exhibited low PA production. LPS-stimulated PA accumulation was inhibited by 1-butanol, which blocks the PLD-catalyzed transphosphatidylation reaction. The increase in TOR expression induced by LPS treatment was also inhibited by 1-butanol. Exogenous application of various PA species (e.g., di16:0-PA, di18:0-PA, and di18:1-PA) increased TOR expression, with unsaturated PA (di18:1-PA) demonstrating the highest biological potency. LPS treatment further increased the expression of ribosomal genes RPS27B (Small Ribosomal Subunit Protein 27B) and RPL7B (Large Ribosomal Subunit Protein L7B), but not RPS6B (Ribosomal Protein 6S). The expression of the cell cycle reporter CycB1;1::GUS in the root meristem, a process influenced by TOR, was stimulated by the application of different PA species, with di18:1-PA showing the strongest effect. These results highlights the role of PLD activity as a modulator of Arabidopsis TOR signaling in response to A. baldaniorum Sp245 LPS
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|a Journal Article
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|a Phosphatidic acid
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|a Phospholipase D
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|a cell division
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|a lipopolysaccharides
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|a root meristem
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|a Phospholipase D
|2 NLM
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|a EC 3.1.4.4
|2 NLM
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|a Lipopolysaccharides
|2 NLM
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|a Arabidopsis Proteins
|2 NLM
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|a Phosphatidic Acids
|2 NLM
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|a TOR Serine-Threonine Kinases
|2 NLM
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|a EC 2.7.11.1
|2 NLM
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| 650 |
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|a TOR protein, Arabidopsis
|2 NLM
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| 650 |
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|a EC 2.7.1.137
|2 NLM
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| 650 |
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|a Phosphatidylinositol 3-Kinases
|2 NLM
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| 650 |
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|a EC 2.7.1.-
|2 NLM
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|a Castro-Mercado, Elda
|e verfasserin
|4 aut
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|a Flores-Cortez, Idolina
|e verfasserin
|4 aut
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|a Valencia-Cantero, Eduardo
|e verfasserin
|4 aut
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|a López-Bucio, José
|e verfasserin
|4 aut
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|a Reyes-de la Cruz, Homero
|e verfasserin
|4 aut
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|a García-Pineda, Ernesto
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 359(2025) vom: 05. Aug., Seite 112653
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnas
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| 773 |
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|g volume:359
|g year:2025
|g day:05
|g month:08
|g pages:112653
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|u http://dx.doi.org/10.1016/j.plantsci.2025.112653
|3 Volltext
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