U-box E3 ubiquitin ligase OsPUB41 represses rice blast resistance by promoting degradation of OsPALs

U-box E3 ubiquitin ligase OsPUB41 represses rice blast resistance by promoting degradation of OsPALs

© 2025 The Author(s). New Phytologist © 2025 New Phytologist Foundation.

Détails bibliographiques
Publié dans:The New phytologist. - 1979. - 247(2025), 5 vom: 01. Aug., Seite 2312-2327
Auteur principal: Xu, Li (Auteur)
Autres auteurs: Tang, Yongyan, Wang, Mei, Peng, Xuan, Shi, Hui, Zhang, Zhenyu, Zhang, Yanling, Yin, Junjie, Xiong, Qing, Lu, Xiang, Zhou, Yang, Chen, Xuewei, Wang, Jing
Format: Article en ligne
Langue:English
Publié: 2025
Accès à la collection:The New phytologist
Sujets:Journal Article E3 ubiquitin ligase OsPAL1 OsPUB41 blast resistance plant U‐box protein rice Ubiquitin-Protein Ligases EC 2.3.2.27 Plant Proteins plus... Lignin 9005-53-2 Phenylalanine Ammonia-Lyase EC 4.3.1.24
Description
Résumé:© 2025 The Author(s). New Phytologist © 2025 New Phytologist Foundation.
Plant U-box (PUB) proteins, the smallest E3 ubiquitin ligase subfamily, play key roles in plant growth, development, and responses to biotic/abiotic stresses. However, their functions in rice immunity remain largely unexplored. Here, we identified Oryza sativa plant U-box 41 (OsPUB41) as a blast-induced PUB gene via transcriptome analysis and quantitative polymerase chain reaction detection. And the role of OsPUB41 in blast resistance was investigated using both OsPUB41 knockout and over-expression transgenic plants. OsPUB41 functions as an active E3 ligase, promoting the degradation of Oryza sativa phenylalanine ammonia-lyase 1 (OsPAL1) and its homologous proteins, which are key enzymes in lignin biosynthesis, via the ubiquitin-proteasome system. Knocking out OsPUB41 elevated OsPALs protein levels, increased lignin accumulation, and strengthened cell walls, thereby limiting Magnaporthe oryzae invasion. Consequently, OsPUB41 knockout enhanced resistance to rice blast without yield penalty, whereas OsPUB41 over-expression reduced resistance to rice blast. Our study uncovers a novel PUB-mediated immune mechanism in rice and proposesOsPUB41 as a potential target for (CRISPR/Cas9)-mediated genome editing, which may facilitate the development of blast-resistant rice without compromising agronomic traits
Description:Date Completed 07.08.2025
Date Revised 07.08.2025
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.70354