Cooperative effect of N2H4 on anaerobic ammonium-oxidizing bacteria and heterotrophic bacteria and the underlying mechanism
© 2025 The Authors This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC BY-NC 4.0), which permits copying, adaptation and redistribution for non-commercial purposes, provided the original work is properly cited (http://creativecommons.org/license...
| Publié dans: | Water science and technology : a journal of the International Association on Water Pollution Research. - 1986. - 92(2025), 2 vom: 31. Juli, Seite 285-300 |
|---|---|
| Auteur principal: | |
| Autres auteurs: | , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2025
|
| Accès à la collection: | Water science and technology : a journal of the International Association on Water Pollution Research |
| Sujets: | Journal Article anaerobic ammonia oxidation cooperative mechanism heterotrophic flora hydrazine Ammonium Compounds Hydrazines 27RFH0GB4R Nitrogen N762921K75 |
| Résumé: | © 2025 The Authors This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC BY-NC 4.0), which permits copying, adaptation and redistribution for non-commercial purposes, provided the original work is properly cited (http://creativecommons.org/licenses/by-nc/4.0/). Batch experiments investigated cooperative nitrogen removal between anaerobic ammonium-oxidizing bacteria (AnAOB) and heterotrophs under hydrazine (N2H4) and sodium acetate effects. A single addition of sodium acetate (optimal C/N = 2) or N2H4 (1 mg/L) achieved peak total nitrogen removal efficiency of 89.74 and 79.95%, respectively, showing initial enhancement followed by decline with increasing dosage. Combined additions exhibited a V-shaped efficiency trend, with the NH01_CN2 group (C/N = 2, N2H4 = 1 mg/L) achieving 76.76% removal efficiency. Appropriate carbon-to-nitrogen (C/N) ratios allowed hydrazine to promote synergistic interactions. These interactions improved the activity of critical nitrogen-converting genes. The enhanced genes included nitrite oxidoreductase (EC 1.7.99.-), nitrate reductase (EC 1.7.5.1), and cytochrome nitrate reductase (EC 1.9.6.1). Metagenomic analysis revealed that AnAOB primarily engaged in core metabolic pathways, while heterotrophs dominated nucleotide metabolism. Exogenous additives induced metabolic shifts in AnAOB toward versatile environmental adaptations. These findings elucidate the carbon-nitrogen coordination mechanisms governing AnAOB-heterotroph partnerships during nitrogen cycle optimization |
|---|---|
| Description: | Date Completed 31.07.2025 Date Revised 31.07.2025 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 0273-1223 |
| DOI: | 10.2166/wst.2025.087 |