Positional 13C enrichment analysis of aspartate determines PEPC activity in vivo
© 2025 The Author(s). New Phytologist © 2025 New Phytologist Foundation.
| Publié dans: | The New phytologist. - 1979. - 248(2025), 1 vom: 05. Sept., Seite 401-414 |
|---|---|
| Auteur principal: | |
| Autres auteurs: | , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2025
|
| Accès à la collection: | The New phytologist |
| Sujets: | Journal Article CO2 assimilation C‐positional E13C analysis GC‐MS PEPC RUBISCO Synechocystis sp. PCC 6803 aspartate dynamic 13C labeling Carbon Isotopes plus... |
| Résumé: | © 2025 The Author(s). New Phytologist © 2025 New Phytologist Foundation. Photoautotrophic organisms fix inorganic carbon (Ci) by RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE (RUBISCO) and PHOSPHOENOLPYRUVATE CARBOXYLASE (PEPC). Monitoring Ci assimilation rates in vivo is a major challenge in analyzing photoautotrophic metabolism and engineering improved photosynthesis, as conventional methods do not distinguish between these two fluxes. We explored widely applied gas chromatography mass spectrometry (GC-MS) metabolite profiling for C-positional fractional 13C enrichment (E13C) analyses of aspartate to differentiate within one molecule between PEPC, RUBISCO, and CBB cycle activities by 13C pulse-labeling. We validated this method using two GC-MS instruments and two prevailing chemical derivatization methods. We selectively determined E13C at each carbon position of aspartate with accuracy < 1% and precision < 2.5%. In combination with dynamic 13CO2 labeling of Synechocystis cultures, we determined PEPC activity in vivo alongside assessments of RUBISCO and CBB cycle activities. We demonstrate that RUBISCO is inactive in the dark, whereas PEPC remains active but at a lower rate than during the day. Accurate quantifications of aspartate concentrations and positional E13Cs provide molar Ci assimilation rates of photoautotrophic Synechocystis cultures. This technology can be combined with C-positional analyses of other metabolites, for example 3-phosphoglycerate, and may be adapted to characterize natural and biosynthetically engineered Ci-assimilation |
|---|---|
| Description: | Date Completed 04.09.2025 Date Revised 06.09.2025 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.70412 |