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250724s2025 xx |||||o 00| ||eng c |
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|a 10.1016/j.wasman.2025.115023
|2 doi
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|a pubmed25n1527.xml
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|a (DE-627)NLM389981427
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|a (NLM)40700789
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|a (PII)S0956-053X(25)00434-9
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|a DE-627
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|e rakwb
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|a eng
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|a Tomaszewska-Hetman, Ludwika
|e verfasserin
|4 aut
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|a Enhanced conversion of crude glycerol to α-ketoglutaric acid by wild-type Yarrowia lipolytica through feeding strategy optimization
|b An environmentally sustainable approach to industrial waste valorization
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|c 2025
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 10.08.2025
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|a Date Revised 10.08.2025
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2025 The Author(s). Published by Elsevier Ltd.. All rights reserved.
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|a The aim of this study was to develop a microbiological process enabling the efficient valorization of crude glycerol into α-ketoglutaric acid. The study focused on assessing the effect of thiamine availability and substrate feeding strategy on α-ketoglutarate biosynthesis by the wild-type strain Yarrowia lipolytica A-8. The bioreactor cultures were performed in a fed-batch system, under thiamine growth-limiting conditions, using both pure and crude glycerol (300 g/L) as sole carbon sources. Optimization of thiamine level was found to be a key factor reducing pyruvic acid by-production, while modifying the substrate dosing strategy, from pulse to continuous feeding, allowed erythritol formation to be limited. Crude glycerol application supported enhanced biomass growth and α-ketoglutaric acid production. A continuous feeding strategy of crude substrate combined with optimized thiamine supplementation (20 μg/L) resulted in relatively high biomass growth (∼40 g/L) while maintaining high α-ketoglutarate production efficiency and selectivity (up to 95 %). The best results were obtained in the process where 50 g/L of crude glycerol was present in the initial medium, followed by continuous feeding of 250 g/L at a constant rate of 4.6 g/L/h starting from the 24th hour of cultivation. In these conditions, α-ketoglutarate production reached 117.7 g/L, with volumetric productivity of 0.81 g/L/h and a yield of 0.39 g/g, surpassing values obtained with pure glycerol. Thus, the proposed process offers a sustainable waste-to-resource solution for crude glycerol utilization
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|a Journal Article
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|a Biodiesel waste
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|a Crude glycerol
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|a Valorization
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|a Yarrowia lipolytica
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|a α-ketoglutaric acid
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|a Glycerol
|2 NLM
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|a PDC6A3C0OX
|2 NLM
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|a Ketoglutaric Acids
|2 NLM
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|a Industrial Waste
|2 NLM
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|a Thiamine
|2 NLM
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|a X66NSO3N35
|2 NLM
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|a Rywińska, Anita
|e verfasserin
|4 aut
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|a Wiśniewska, Julia
|e verfasserin
|4 aut
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| 700 |
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|a Rymowicz, Waldemar
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Waste management (New York, N.Y.)
|d 1999
|g 205(2025) vom: 23. Aug., Seite 115023
|w (DE-627)NLM098197061
|x 1879-2456
|7 nnas
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| 773 |
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|g volume:205
|g year:2025
|g day:23
|g month:08
|g pages:115023
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|u http://dx.doi.org/10.1016/j.wasman.2025.115023
|3 Volltext
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