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250718s2025 xx |||||o 00| ||eng c |
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|a 10.1016/j.plantsci.2025.112672
|2 doi
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|a pubmed25n1528.xml
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|a (DE-627)NLM389629170
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|a (NLM)40675382
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|a (PII)S0168-9452(25)00290-0
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Song, Jieqiong
|e verfasserin
|4 aut
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|a SlXTH1, a xyloglucan endotransglucosylase/hydrolase in halophyte Suaeda liaotungensis, negatively regulates Arabidopsis seed germination under salt stress
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|c 2025
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 12.08.2025
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|a Date Revised 12.08.2025
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2025 Elsevier B.V. All rights reserved.
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|a Seed germination is accompanied by cell wall remodeling. Cell wall remodeling proteins, xyloglucan endotransglucosylase/hydrolase (XTHs), play a crucial role in embryo cell growth and endosperm weakening during seed germination. Nonetheless, the function of XTHs in seed germination under salt stress has not been reported. In this study, a novel XTH gene, designated SlXTH1 from halophyte Suaeda liaotungensis was identified. The predicted SlXTH1 protein contained an N-terminal signal peptide and catalytic conserved motif DEIDFEFLG, N-glycosylation sites and cysteine residues in the C-terminal region. Phylogenetic analysis showed that SlXTH1 belonged to group Ⅰ/Ⅱ exhibiting xyloglucan endotransglucosylase (XET) activity. Transient expression assays in onion cell revealed that SlXTH1 was localized to cell wall. Expression pattern analysis of SlXTH1 gene revealed that expressed in germinated seed and response to ABA and salt stress. Tissue-specific localization revealed SlXTH1 mRNA accumulation in the radicle and embryonic axis of germinated seeds. SlXTH1 negatively regulated seed germination under salt stress, as evidenced by reduced germination rates, elevated H2O2 and MDA content and diminished activities of antioxidant enzymes. Overexpression of SlXTH1 significantly elevated XET activity and increased xyloglucan and cellulose content, resulting in cell wall remodeling during seed germination under salt stress. Taken together, these results demonstrate that SlXTH1 acts as a negative regulator during germination under salt stress
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|a Journal Article
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|a Cell wall
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|a Salt stress
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|a Seed germination
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|a Suaeda liaotungensis
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|a XTHs
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|a Glycosyltransferases
|2 NLM
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| 650 |
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|a EC 2.4.-
|2 NLM
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| 650 |
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|a xyloglucan - xyloglucosyltransferase
|2 NLM
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| 650 |
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|a EC 2.4.1.207
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 700 |
1 |
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|a Ding, Haoran
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Cheng, Nuo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Xinxin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lu, Yanke
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Hongfei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Changping
|e verfasserin
|4 aut
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| 700 |
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|a Li, Qiuli
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant science : an international journal of experimental plant biology
|d 1985
|g 359(2025) vom: 15. Aug., Seite 112672
|w (DE-627)NLM098174193
|x 1873-2259
|7 nnas
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| 773 |
1 |
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|g volume:359
|g year:2025
|g day:15
|g month:08
|g pages:112672
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| 856 |
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|u http://dx.doi.org/10.1016/j.plantsci.2025.112672
|3 Volltext
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|d 359
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|h 112672
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