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01000naa a22002652c 4500 |
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NLM389204943 |
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DE-627 |
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20250714160844.0 |
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250714s2025 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-04-25-0837-PDN
|2 doi
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|a pubmed25n1426.xml
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|a eng
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| 100 |
1 |
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|a Gao, Ying
|e verfasserin
|4 aut
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|a First report of Erysiphe heraclei causing powdery mildew on cilantro in Tennessee and the United States
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|c 2025
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|a Text
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Revised 28.05.2025
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|a published: Print-Electronic
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|a Citation Status Publisher
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|a Cilantro, Coriandrum sativum L., is an important culinary herb within the family of Apiaceae. Cilantro, with its fragrant, citrusy flavour and medicinal uses, has been cultivated commercially under protected environments in several U.S. states including Tennessee. In March 2025 (early spring), we observed the typical symptom of powdery mildew, white powdery fungal growth, on leaves and stems of cilantro cultivated in two commercial green houses in Eagleville, TN. A disease incidence of 78% was recorded from 250 randomly selected cilantro plants across 25 rows. The disease severity was estimated at 54% based on a 0-5 scale disease rating (Behera et al., 2024). Microscopic examination of the fungal pathogen revealed epiphytic hyaline mycelia and upright unbranched conidiophores measuring 83-107 × 7.70-11.35 μm, which arose from cylindrical slightly curved foot cells measuring 26-43 × 8-13 μm. Conidia were ellipsoid to ovoid, produced singly and measured 24-47 × 11-16 μm. No chasmothecia, the sexual fruiting bodies, were observed in the infected samples. Based on these morphological characteristics, the pathogen was identified as Erysiphe heraclei. To further confirm identification, genomic DNA was extracted from conidiophores and conidia and the internal transcribed spacer (ITS) region was amplified using primers ITS1 and ITS4 (White et al., 1990). A ~700 bp amplicon was obtained and sequenced bidirectionally. The resulting sequence (Acc. No. PV489566) showed 100% similarity with E. heraclei and E. betae, which have been previously reported on cilantro in Mexico (LC777887) and on Swiss chard in South Africa (PQ220062), respectively. Based on the host specificity of E. heraclei, which is known to infect only plants within the Apiaceae family (Braun, 2012), the pathogen associated with powdery mildew on cilantro was confirmed as E. heraclei. Koch's postulates were further confirmed through rub inoculation, whereby infected leaves were gently rubbed onto the healthy leaves of 30-day-old cilantro plants [2-3 plants per pot; (n = 10 pots)] and maintained under greenhouse conditions at 22 °C with a 16:8 h light and dark cycle. Six days post-inoculation, characteristic powdery mildew symptoms, white powdery patches, appeared on the treated leaves while no symptoms were observed in the uninoculated control plants (n = 3 pots). Microscopic and molecular analyses of the fungi on inoculated plants matched that of the original isolate. Based on both morphological and molecular analyses, the fungal pathogen causing powdery mildew on cilantro was confirmed as E. heraclei. To the best of our knowledge, this is the first documented occurrence of E. heraclei infecting cilantro in the United States. Therefore, implementing appropriate preventive measures is crucial to minimize potential yield losses, particularly under protected cultivation conditions
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|a Journal Article
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|a Erysiphe heraclei
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| 650 |
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4 |
|a cilantro
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| 650 |
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|a molecular
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| 650 |
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4 |
|a morphometric
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| 650 |
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4 |
|a powdery mildew
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| 700 |
1 |
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|a Chinnaiah, Senthilraja
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g (2025) vom: 28. Mai
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnas
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| 773 |
1 |
8 |
|g year:2025
|g day:28
|g month:05
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| 856 |
4 |
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|u http://dx.doi.org/10.1094/PDIS-04-25-0837-PDN
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