Comprehensive structural, evolutionary and functional analysis of superoxide dismutase gene family revealed critical role in salinity and drought stress responses in chickpea (Cicer arietinum L.)
Copyright © 2025 Elsevier Masson SAS. All rights reserved.
| Veröffentlicht in: | Plant physiology and biochemistry : PPB. - 1991. - 226(2025) vom: 21. Mai, Seite 110042 |
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| Weitere Verfasser: | , , , , , , , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2025
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| Zugriff auf das übergeordnete Werk: | Plant physiology and biochemistry : PPB |
| Schlagworte: | Journal Article Antioxidant defence CaSODs Chickpea Drought stress Gene expression Salinity stress |
| Zusammenfassung: | Copyright © 2025 Elsevier Masson SAS. All rights reserved. Superoxide dismutase (SOD), a metalloenzyme, catalyses the dismutation of superoxide anions (O2•‾) into molecular oxygen (O2) and hydrogen peroxide (H2O2), perform crucial roles in plant growth, development, and responses to multiple abiotic stressors. Present study attempted to explore the SOD gene family in chickpea and their key role in salinity and drought tolerance. Computational analysis of SOD gene family in chickpea revealed 10 SODs (4 Cu/ZnSODs and 6 Mn/FeSODs) and explored their chromosomal location, evolutionary relationships, structure, conserved motifs, promoter analysis, tissue specific expression analysis, protein-protein interactions and docking of CaSODs with their predicted interacting partners. GO (gene ontology) and KEGG analysis revealed association of CaSODs in ROS signalling, metal binding, and catalysis, which contribute in stress tolerance and cellular homeostasis. Further, transcriptomic analysis revealed that CaSODs showed differential expression pattern under salinity and drought conditions. qRT-PCR was performed to analyse the response of CaSODs in salinity ICCV2 (tolerant), JG62 (susceptible) and drought ICC4958 (tolerant), ICC1882 (susceptible) genotypes. A comparative analysis of gene expression in ICCV2, JG62, ICC4958 and ICC1882 revealed number of CaSODs, such as CaCSD3, CaCSD2, and CaCSD4, showed high expression in response to salinity and drought stress, suggesting their involvement in stress response pathways as predicted by GO analysis. miRNA analysis revealed that CaCSDs and CaMSDs were targeted by miRNAs (CaCSD4-miR398a/b/c, and CaMSD-miR747). Additionally, the study found SNP variation in two CaSODs (CaMSD5 and CaMSD6) promoter regions, which could affect expression pattern of these genes. Our findings provide the basis to understand the functional roles of CaCSD3/CaCSD4 in salinity tolerance and CaCSD3 for drought tolerance by reducing oxidative stress, offer important information for future research with the objective of improving chickpea stress tolerance using breeding or genetic engineering technologies |
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| Beschreibung: | Date Revised 25.05.2025 published: Print-Electronic Citation Status Publisher |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2025.110042 |