Integrated transcriptome and metabolome analysis revealed the molecular regulatory mechanism of carbohydrate synthesis in Panax ginseng
Copyright © 2025. Published by Elsevier B.V.
| Publié dans: | Plant science : an international journal of experimental plant biology. - 1985. - (2025) vom: 20. Mai, Seite 112573 |
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| Auteur principal: | |
| Autres auteurs: | , , , |
| Format: | Article en ligne |
| Langue: | English |
| Publié: |
2025
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| Accès à la collection: | Plant science : an international journal of experimental plant biology |
| Sujets: | Journal Article Monosaccharides Panax ginseng Polysaccharides Potassium fertilizers Transcriptome and metabolome |
| Résumé: | Copyright © 2025. Published by Elsevier B.V. Ginseng (Panax ginseng) is renowned for its medicinal properties, which are primarily attributed to bioactive compounds such as polysaccharides and secondary metabolites. However, the mechanisms underlying carbohydrate synthesis and metabolism in ginseng remains poorly understood. In this study, we investigated the effects of different potassium fertilizers, including potassium fulvic acid (BSFA), potassium humate (KHM), and potassium sulfate (KS), on the accumulation of monosaccharides and polysaccharides in ginseng. Through integrated metabolomic and transcriptomic analyses, we elucidated the metabolic pathways and gene networks associated with monosaccharide and polysaccharide biosynthesis under these treatments. Among the tested fertilizers, KS treatment significantly enhanced the accumulation of galactose, arabinose, and crude polysaccharides, outperforming both BSFA and KHM treatments. Metabolomic profiling revealed distinct metabolic reprogramming induced by each treatment: BSFA and KHM treatments enriched pathways related to phosphatidylinositol signaling and autophagy, whereas KS treatment uniquely activated monoterpenoid biosynthesis. Transcriptomic analysis further demonstrated that KS treatment markedly upregulated key genes involved in nucleotide sugar metabolism, including EVM0043355 (UAE), EVM0012792 (UDP-D-xylose synthase), and EVM0013821 (UDP-D-xylose synthase), as well as starch hydrolysis-related genes such as EVM0053524 (glycosyl hydrolase family) and EVM0000395 (hexokinase). Notably, UDP-D-xylose synthase plays a pivotal role in generating polysaccharide precursors, and the nucleotide sugar metabolism pathway may regulate the diversion of sugar intermediates toward polysaccharide biosynthesis. These findings further support the role of KS in promoting polysaccharide synthesis. Network analysis identified critical gene-metabolite interactions, highlighting the importance of nucleotide sugar metabolism in polysaccharide biosynthesis. Collectively, our results provide novel insights into how potassium fertilizers modulate ginseng's metabolic profile and offer practical strategies for optimizing cultivation practices to improve its medicinal value |
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| Description: | Date Revised 22.05.2025 published: Print-Electronic Citation Status Publisher |
| ISSN: | 1873-2259 |
| DOI: | 10.1016/j.plantsci.2025.112573 |