Genome wide identification and functional analyses of HAK family potassium transporter genes in passion fruit (Passiflora edulis Sims) in response to potassium deficiency and stress responses
Copyright © 2025 Elsevier Masson SAS. All rights reserved.
| Veröffentlicht in: | Plant physiology and biochemistry : PPB. - 1991. - 225(2025) vom: 10. Mai, Seite 109995 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2025
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| Zugriff auf das übergeordnete Werk: | Plant physiology and biochemistry : PPB |
| Schlagworte: | Journal Article Gene expression profiles Gene functions HAK family Passiflora edulis Potassium stress Stress responses |
| Zusammenfassung: | Copyright © 2025 Elsevier Masson SAS. All rights reserved. The nutritional status of potassium directly affects the yield and quality of fruits. The molecular mechanism underlying K+ uptake and transport in passion fruit (Passiflora edulis Sims), particularly under K+ limited conditions, remains poorly understood. Members of the high-affinity K+ (HAK) transporter family play a vital role in K+ acquisition, translocation, and stress responses. However, the biological functions of these genes in passion fruit plants are still unknown. This study identified 14 HAK genes (PeHAKs) in Passiflora edulis genome. Phylogenetic analysis classified these PeHAKs into three distinct clusters containing 9, 4, and 1 genes, respectively, with conserved structural features supporting their functional divergence. Promoter analysis revealed 12 predominant cis-acting elements, including hormone-responsive, stress-inducible, and core transcriptional regulatory motifs. Tissue-specific expression profiling demonstrated significant organ-dependent expression patterns of PeHAKs across roots, stems, leaves (young/mature), flowers, and fruits. Under K+ deficiency, salinity stress, and phytohormone treatments, the transcript levels of PeHAKs were significantly altered in roots and leaves. Notably, PeHAK10 exhibited dual induction in aerial and subterranean tissues under K+ deprivation. Functional complementation assays in yeast validated the K+/Na + transport activity of PeHAK10, suggesting its involvement in ion homeostasis regulation during nutrient stress. This study provides the first genome-wide characterization of the PeHAKs family of genes in passion fruit plants, establishing a foundation for elucidating their biological roles in potassium nutrition regulation and stress adaptation |
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| Beschreibung: | Date Revised 17.05.2025 published: Print-Electronic Citation Status Publisher |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2025.109995 |