Transcription factor OsNAC29a confers drought tolerance through the ABA pathway in rice
Copyright © 2025 Elsevier Masson SAS. All rights reserved.
| Veröffentlicht in: | Plant physiology and biochemistry : PPB. - 1991. - 225(2025) vom: 07. Mai, Seite 109989 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2025
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| Zugriff auf das übergeordnete Werk: | Plant physiology and biochemistry : PPB |
| Schlagworte: | Journal Article ABA pathway Drought tolerance NAC transcription factor OsNAC29a OsPOD1 Rice (Oryza sativa L.) |
| Zusammenfassung: | Copyright © 2025 Elsevier Masson SAS. All rights reserved. The plant-specific NAC transcription factor family plays a crucial role in mediating responses to abiotic stress, but the functions of many NAC genes remain poorly characterized. The rice OsNAC29a gene is induced by PEG and abscisic acid (ABA). OsNAC29a exhibits transactivation activity and the region of 248-315 amino acids at its C-terminus is essential for its activation. Over-expression of OsNAC29a enhances drought resistance and ABA sensitivity in transgenic rice. OsNAC29a over-expression modulates physiological indicators related to stress resistance, while RNAi-mediated down-regulation of OsNAC29a results in opposite phenotypes and physiological changes. Under drought conditions, OsNAC29a over-expression significantly up-regulates stress-related genes such as OsP5CS1, OsSRO1c, OsPOD1, OsLEA3, and OsRab16C. Interestingly, OsPOD1 gene expression increases in OsNAC29a over-expression rice under both normal and drought stress conditions, leading to significantly enhanced peroxidase activity. Further research reveals that OsNAC29a binds to the OsPOD1 promoter to drive its expression. Additionally, OsSAPK2 which is a key component of the ABA-dependent drought-tolerance pathway interacts physically with OsNAC29a and enhances its transcriptional activation activity. Collectively, acting as a positive regulator of drought tolerance, OsNAC29a regulates drought resistance in rice by directly or indirectly modulating stress-responsive genes |
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| Beschreibung: | Date Revised 17.05.2025 published: Print-Electronic Citation Status Publisher |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2025.109989 |