The Cytokinin Oxidase/Dehydrogenase TaCKX11-D Positively Regulates Grain Size in Wheat

© The Author(s) 2025. Published by Oxford University Press on behalf of Society for Experimental Biology. All rights reserved. For commercial re-use, please contact reprintsoup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service...

Ausführliche Beschreibung

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - (2025) vom: 13. Mai
1. Verfasser: Qian, Qian (VerfasserIn)
Weitere Verfasser: Hu, Weiguo, Bi, Zhenzhen, Li, Limin, Wang, Keke, Zhang, Xiaohong, Min, Donghong
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2025
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article TaCKX11-D Triticum aestivum Cytokinin oxidase/dehydrogenase Grain size Protein interaction Transgenic plant
LEADER 01000naa a22002652c 4500
001 NLM388428112
003 DE-627
005 20250714082845.0
007 cr uuu---uuuuu
008 250714s2025 xx |||||o 00| ||eng c
024 7 |a 10.1093/jxb/eraf196  |2 doi 
028 5 2 |a pubmed25n1404.xml 
035 |a (DE-627)NLM388428112 
035 |a (NLM)40357640 
035 |a (PII)eraf196 
040 |a DE-627  |b ger  |c DE-627  |e rakwb 
041 |a eng 
100 1 |a Qian, Qian  |e verfasserin  |4 aut 
245 1 4 |a The Cytokinin Oxidase/Dehydrogenase TaCKX11-D Positively Regulates Grain Size in Wheat 
264 1 |c 2025 
336 |a Text  |b txt  |2 rdacontent 
337 |a ƒaComputermedien  |b c  |2 rdamedia 
338 |a ƒa Online-Ressource  |b cr  |2 rdacarrier 
500 |a Date Revised 13.05.2025 
500 |a published: Print-Electronic 
500 |a Citation Status Publisher 
520 |a © The Author(s) 2025. Published by Oxford University Press on behalf of Society for Experimental Biology. All rights reserved. For commercial re-use, please contact reprintsoup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com. 
520 |a Grain size is one of the essential determinants of yield in wheat (Triticum aestivum L.). Cytokinin signaling affects grain size, and cytokinin oxidase/dehydrogenase (CKX) regulates cytokinin homeostasis by catalyzing cytokinin degradation. In this study, we identified that TaCKX11-D positively regulates wheat grain size. Overexpression of TaCKX11-D significantly increased grain length, grain width, grain thickness, and grain weight in Arabidopsis and wheat, whereas silencing TaCKX11-D in wheat decreased grain size and grain weight. Cytological analysis revealed that TaCKX11-D affects wheat grain size by regulating outer pericarp cell size. Overexpression of TaCKX11-D was determined to reduce endogenous cytokinin content in wheat. This demonstrates that TaCKX11-D promotes cell expansion and increases grain size by regulating cytokinin homeostasis. Moreover, we discovered that TaCKX11-D directly interacts with and is phosphorylated by TaMPK3 and TaMPK6. Our results reveal that TaCKX11-D positively regulates grain size and provide insights for wheat improvement breeding 
650 4 |a Journal Article 
650 4 |a TaCKX11-D 
650 4 |a Triticum aestivum 
650 4 |a Cytokinin oxidase/dehydrogenase 
650 4 |a Grain size 
650 4 |a Protein interaction 
650 4 |a Transgenic plant 
700 1 |a Hu, Weiguo  |e verfasserin  |4 aut 
700 1 |a Bi, Zhenzhen  |e verfasserin  |4 aut 
700 1 |a Li, Limin  |e verfasserin  |4 aut 
700 1 |a Wang, Keke  |e verfasserin  |4 aut 
700 1 |a Zhang, Xiaohong  |e verfasserin  |4 aut 
700 1 |a Min, Donghong  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Journal of experimental botany  |d 1985  |g (2025) vom: 13. Mai  |w (DE-627)NLM098182706  |x 1460-2431  |7 nnas 
773 1 8 |g year:2025  |g day:13  |g month:05 
856 4 0 |u http://dx.doi.org/10.1093/jxb/eraf196  |3 Volltext 
912 |a GBV_USEFLAG_A 
912 |a SYSFLAG_A 
912 |a GBV_NLM 
912 |a GBV_ILN_350 
951 |a AR 
952 |j 2025  |b 13  |c 05