The Cytokinin Oxidase/Dehydrogenase TaCKX11-D Positively Regulates Grain Size in Wheat
© The Author(s) 2025. Published by Oxford University Press on behalf of Society for Experimental Biology. All rights reserved. For commercial re-use, please contact reprintsoup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service...
| Veröffentlicht in: | Journal of experimental botany. - 1985. - (2025) vom: 13. Mai |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2025
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| Zugriff auf das übergeordnete Werk: | Journal of experimental botany |
| Schlagworte: | Journal Article TaCKX11-D Triticum aestivum Cytokinin oxidase/dehydrogenase Grain size Protein interaction Transgenic plant |
| Zusammenfassung: | © The Author(s) 2025. Published by Oxford University Press on behalf of Society for Experimental Biology. All rights reserved. For commercial re-use, please contact reprintsoup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com. Grain size is one of the essential determinants of yield in wheat (Triticum aestivum L.). Cytokinin signaling affects grain size, and cytokinin oxidase/dehydrogenase (CKX) regulates cytokinin homeostasis by catalyzing cytokinin degradation. In this study, we identified that TaCKX11-D positively regulates wheat grain size. Overexpression of TaCKX11-D significantly increased grain length, grain width, grain thickness, and grain weight in Arabidopsis and wheat, whereas silencing TaCKX11-D in wheat decreased grain size and grain weight. Cytological analysis revealed that TaCKX11-D affects wheat grain size by regulating outer pericarp cell size. Overexpression of TaCKX11-D was determined to reduce endogenous cytokinin content in wheat. This demonstrates that TaCKX11-D promotes cell expansion and increases grain size by regulating cytokinin homeostasis. Moreover, we discovered that TaCKX11-D directly interacts with and is phosphorylated by TaMPK3 and TaMPK6. Our results reveal that TaCKX11-D positively regulates grain size and provide insights for wheat improvement breeding |
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| Beschreibung: | Date Revised 13.05.2025 published: Print-Electronic Citation Status Publisher |
| ISSN: | 1460-2431 |
| DOI: | 10.1093/jxb/eraf196 |