Four guidelines to identify bona fide protease-substrate pairs
© The Author(s) 2025. Published by Oxford University Press on behalf of Society for Experimental Biology.
| Veröffentlicht in: | Journal of experimental botany. - 1985. - (2025) vom: 09. Mai |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2025
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| Zugriff auf das übergeordnete Werk: | Journal of experimental botany |
| Schlagworte: | Journal Article Activity-based protein profiling probe (ABPP) N-terminomics Plant proteases Protease inhibitors Protease substrates Proteolysis Substrate cleavage site |
| Zusammenfassung: | © The Author(s) 2025. Published by Oxford University Press on behalf of Society for Experimental Biology. Plant proteases are an important class of enzymes, with proposed involvement in various aspects of the plant life cycle. However, pinpointing authentic protease-substrate interactions remains challenging, which hinders a comprehensive understanding of the biological function of proteases. Moreover, a structured set of guidelines to validate protease substrates is lacking. In this review, we outline a minimum of four key guidelines that, when followed, can confirm the specificity of protease-substrate interaction for proteases that perform limited proteolysis and with specific cleavage sites: (i) the observation of substrate cleavage; (ii) the reduction in substrate cleavage due to protease inhibitors or (iii) genetic mutation of the protease; (iv) a final proof of the specificity of the substrate cleavage site. It is important to emphasize that these guidelines are not universally applicable to all proteases. By creating a set of guidelines, summarizing current findings and proposing future research directions, this review aims to highlight innovative techniques that will improve the specificity and accuracy of protease research and facilitate a deeper understanding of the role of proteases in plant biology |
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| Beschreibung: | Date Revised 09.05.2025 published: Print-Electronic Citation Status Publisher |
| ISSN: | 1460-2431 |
| DOI: | 10.1093/jxb/eraf194 |