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250509s2025 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/eraf113
|2 doi
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|a pubmed25n1362.xml
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|a (DE-627)NLM388161914
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|a (NLM)40168132
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|a (PII)eraf113
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Li, Da
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Calmodulin1 (CsCAM1) - Calmodulin binding transcription activator (CsCAMTA) negatively regulates the transcription of fluoride export gene CsFEX1 to mediate fluoride transport in tea plants (Camellia sinensis)
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|c 2025
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Revised 01.04.2025
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|a published: Print-Electronic
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|a Citation Status Publisher
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|a © The Author(s) 2025. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a Fluoride (F) is vital for tea plant growth; however, high levels of F in tea leaves could be a potential threat to human health. According to previous studies, F export genes (CsFEXs) regulate F efflux from tea plant cells, but the specific regulatory network remains unclear. In this study, the calmodulin binding transcription activator (CsCAMTA) protein was functionally characterized based on yeast one-hybrid screening assay and previous transcriptomics. CsCAMTA is mainly localized in the nucleus and binds to the G-box (CACGTC) cis-element in the CsFEX1 promoter as a transcription inhibitor. Additionally, yeast-two-hybrid assay and bimolecular fluorescence complementation assay showed that calmodulin 1 (CsCAM1) could interact with CsCAMTA to form a protein complex that suppressed CsFEX1 expression. Furthermore, subcellular localization results indicated that CsCAM1 was localized in the nucleus and cytoplasm, suggesting that CsCAM1 may be activated by binding Ca2+ directly in the cytoplasm and subsequent leading to a physical interaction with CsCAMTA. Finally, silencing of CsCAMTA and CsCAM1 genes in tea plants by using antisense oligodeoxynucleotides increased the CsFEX1 expression level and decreased the intracellular F level. Thus, our study elucidated a CsCAM1-CsCAMTA-CsFEX1 module that regulated F export in tea leaves and provided new insights for breeding low-F tea varieties
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|a Journal Article
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|a Camellia sinensis
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| 650 |
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4 |
|a CsCAM1
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4 |
|a CsCAMTA
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| 650 |
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4 |
|a CsFEX1
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| 650 |
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|a fluoride
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| 650 |
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4 |
|a transcription regulation
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| 700 |
1 |
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|a Jin, Ya
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lu, Qin-Hua
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ren, Ning
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Xue-Ying
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Lin-Ying
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hong, Gao-Jie
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Qin-Qin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhao, Yi-Jie
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Ying-Qi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Qing-Sheng
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g (2025) vom: 01. Apr.
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnas
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| 773 |
1 |
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|g year:2025
|g day:01
|g month:04
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/eraf113
|3 Volltext
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|j 2025
|b 01
|c 04
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