ROOT PHOTOTROPISM 2 (RPT2) is a KAT1 potassium channel regulator required for its accumulation

ROOT PHOTOTROPISM 2 (RPT2) is a KAT1 potassium channel regulator required for its accumulation

Copyright © 2025 The Authors. Published by Elsevier Masson SAS.. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 224(2025) vom: 23. Juli, Seite 109922
1. Verfasser: Locascio, Antonella (VerfasserIn)
Weitere Verfasser: Montoliu-Silvestre, Eva, Nieves-Cordones, Manuel, Petsch, Silvia, Fuchs, Anika, Bou, Claudia, Navarro-Martínez, Alejandro, Porcel, Rosa, Andrés-Colás, Nuria, Rubio, Francisco, Mulet, José Miguel, Yenush, Lynne
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2025
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Arabidopsis Guard cells KAT1 Potassium channel ROOT PHOTOTROPISM 2 Arabidopsis Proteins Potassium Channels, Inwardly Rectifying KAT1 protein, Arabidopsis
Beschreibung
Zusammenfassung:Copyright © 2025 The Authors. Published by Elsevier Masson SAS.. All rights reserved.
In plants, inward rectifying potassium channels regulate potassium entry into guard cells and are a key factor controlling stomatal opening. KAT1 is a major inward rectifying potassium channel present in Arabidopsis thaliana guard cell membranes. The identification of regulators of channels like KAT1 is a promising approach for the development of strategies to improve plant drought tolerance. Using a high-throughput Split-ubiquitin screening in yeast, we identified RPT2 (ROOT PHOTOTROPISM 2) as a KAT1 interactor. Here, we present the results of the characterization of this interaction in yeast and plants. Importantly, we also observe increased KAT1-mediated currents in oocytes co-expressing RPT2, suggesting a functional link between the two proteins. Moreover, using stably transformed KAT1-YFP lines, we show that RPT2 is necessary for KAT1 protein accumulation in A. thaliana. Our data suggest an unexpected role for RPT2 in KAT1 post-translational regulation that may represent a novel connection between light signaling and potassium channel activity
Beschreibung:Date Completed 23.05.2025
Date Revised 23.05.2025
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2025.109922