Integrated transcriptomics, metabolomics and physiological analyses reveal the regulatory mechanism of dopamine in Nicotiana tabacum response to cadmium stress
Copyright © 2025 Elsevier Masson SAS. All rights reserved.
| Veröffentlicht in: | Plant physiology and biochemistry : PPB. - 1991. - 224(2025) vom: 12. Juli, Seite 109915 |
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| Weitere Verfasser: | , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2025
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| Zugriff auf das übergeordnete Werk: | Plant physiology and biochemistry : PPB |
| Schlagworte: | Journal Article Amino acids Cadmium Detoxification mechanisms Dopamine Metabolomics Transcriptomics 00BH33GNGH VTD58H1Z2X |
| Zusammenfassung: | Copyright © 2025 Elsevier Masson SAS. All rights reserved. Cadmium (Cd) pollution is a serious threat to many plants. Dopamine (DOPA) can potentially alleviate abiotic stress in plants, however, the effects of DOPA on Cd biotoxicity remain largely elusive. This study explored the beneficial effects of DOPA on Cd tolerance and detoxification in tobacco using transcriptomics and metabolomics combined with physiological and biochemical analyses. The results showed that applying DOPA reduced Cd absorption in tobacco plants, altered its subcellular distribution, decreased the soluble Cd proportion within cells and organelles, and increased the soluble Cd proportion associated with the cell wall. Analysis of Cd chemical forms revealed that DOPA decreased the concentrations of inorganic Cd and water-soluble Cd (with limited mobility), while increased the Cd complexed with pectin, proteins, and phosphate in the cell walls and vacuoles. Furthermore, DOPA enhanced CAT, SOD, POD, APX activity, decreased Cd-induced O2·-, H2O2, and MDA accumulation and damage, and preserved cell wall structural integrity, thereby improved the plant photosynthetic capacity (Fv/Fm, Gs, Tr, Pn). Comprehensive transcriptome and metabolome analyses revealed that DOPA modulated amino acid and lipid metabolism, and upregulated the expression of genes involved in cell wall synthesis (PME, XTH et al.), Cd uptake and detoxification (FROs, HIPPs et al.), as well as light-harvesting protein (Psa, Psb, LHC et al.). This study establishes a theoretical foundation for understanding how DOPA enhances plant tolerance to Cd stress and elucidates the fundamental regulatory mechanisms involved |
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| Beschreibung: | Date Completed 23.05.2025 Date Revised 23.05.2025 published: Print-Electronic Citation Status MEDLINE |
| ISSN: | 1873-2690 |
| DOI: | 10.1016/j.plaphy.2025.109915 |